@article{bbf8467b8c2e4ce3b2a165865a5c3678,
title = "A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells",
abstract = "Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.",
keywords = "Cell line models, Chromosome rearrangement, Cre/LoxP system, Genome editing",
author = "Anna Khabarova and Galina Koksharova and Pavel Salnikov and Polina Belokopytova and Roman Mungalov and Inna Pristyazhnuk and Artem Nurislamov and Maria Gridina and Veniamin Fishman",
note = "Funding: Generation of the plasmid vectors, derivation and expansion of the cell lines, molecular analysis and NGS sequencing were supported by RSF grant #22–14-00247. Computational data analysis was performed on the high-throughput nodes of the Novosibirsk State University (supported by the Ministry of Education and Science of Russian Federation, grant #2019–0546 (FSUS-2020–0040)). {\textcopyright} 2023. The Author(s), under exclusive licence to Springer Nature B.V.",
year = "2023",
month = feb,
day = "26",
doi = "10.1007/s10577-023-09719-7",
language = "English",
volume = "31",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "1",
}