A cookbook for DNase Hi-C

Maria Gridina, Evgeniy Mozheiko, Emil Valeev, Ludmila P. Nazarenko, Maria E. Lopatkina, Zhanna G. Markova, Maria I. Yablonskaya, Viktoria Yu Voinova, Nadezhda V. Shilova, Igor N. Lebedev, Veniamin Fishman

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Background: The Hi-C technique is widely employed to study the 3-dimensional chromatin architecture and to assemble genomes. The conventional in situ Hi-C protocol employs restriction enzymes to digest chromatin, which results in nonuniform genomic coverage. Using sequence-agnostic restriction enzymes, such as DNAse I, could help to overcome this limitation. Results: In this study, we compare different DNAse Hi-C protocols and identify the critical steps that significantly affect the efficiency of the protocol. In particular, we show that the SDS quenching strategy strongly affects subsequent chromatin digestion. The presence of biotinylated oligonucleotide adapters may lead to ligase reaction by-products, which can be avoided by rational design of the adapter sequences. Moreover, the use of nucleotide-exchange enzymes for biotin fill-in enables simultaneous labelling and repair of DNA ends, similar to the conventional Hi-C protocol. These improvements simplify the protocol, making it less expensive and time-consuming. Conclusions: We propose a new robust protocol for the preparation of DNAse Hi-C libraries from cultured human cells and blood samples supplemented with experimental controls and computational tools for the evaluation of library quality.

Язык оригиналаанглийский
Номер статьи15
ЖурналEpigenetics and Chromatin
Том14
Номер выпуска1
DOI
СостояниеОпубликовано - 20 мар 2021

Предметные области OECD FOS+WOS

  • 1.06 БИОЛОГИЧЕСКИЕ НАУКИ
  • 1.06.KM ГЕНЕТИКА И НАСЛЕДСТВЕННОСТЬ
  • 1.06.CQ БИОХИМИЯ И МОЛЕКУЛЯРНАЯ БИОЛОГИЯ

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