5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

D. S. Novopashina, O. A. Semikolenova, A. G. Venyaminova

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › рецензирование

Выдержка

Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

Язык оригиналаанглийский
Название основной публикацииMethods in Molecular Biology
ИздательHumana Press Inc.
Страницы45-56
Число страниц12
Том2063
DOI
СостояниеОпубликовано - 1 янв 2020

Серия публикаций

НазваниеMethods in Molecular Biology
Том2063
ISSN (печатное издание)1064-3745
ISSN (электронное издание)1940-6029

Отпечаток

RNA Probes
Fluorescent Dyes
Oligonucleotides
RNA
Nucleic Acids
pyrene

Цитировать

Novopashina, D. S., Semikolenova, O. A., & Venyaminova, A. G. (2020). 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. В Methods in Molecular Biology (Том 2063, стр. 45-56). (Methods in Molecular Biology; Том 2063). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0138-9_5
Novopashina, D. S. ; Semikolenova, O. A. ; Venyaminova, A. G. / 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. Methods in Molecular Biology. Том 2063 Humana Press Inc., 2020. стр. 45-56 (Methods in Molecular Biology).
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abstract = "Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.",
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Novopashina, DS, Semikolenova, OA & Venyaminova, AG 2020, 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. в Methods in Molecular Biology. том. 2063, Methods in Molecular Biology, том. 2063, Humana Press Inc., стр. 45-56. https://doi.org/10.1007/978-1-0716-0138-9_5

5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. / Novopashina, D. S.; Semikolenova, O. A.; Venyaminova, A. G.

Methods in Molecular Biology. Том 2063 Humana Press Inc., 2020. стр. 45-56 (Methods in Molecular Biology; Том 2063).

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › рецензирование

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AU - Novopashina, D. S.

AU - Semikolenova, O. A.

AU - Venyaminova, A. G.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

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KW - Mismatch detection

KW - Oligo(2′-O-methylribonucleotides)

KW - Pyrene conjugates

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Novopashina DS, Semikolenova OA, Venyaminova AG. 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. В Methods in Molecular Biology. Том 2063. Humana Press Inc. 2020. стр. 45-56. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-0138-9_5

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