5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

D. S. Novopashina; O. A. Semikolenova; A. G. Venyaminova

doi:10.1007/978-1-0716-0138-9_5

5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

D. S. Novopashina, O. A. Semikolenova, A. G. Venyaminova

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › рецензирование

Аннотация

Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

Язык оригинала	английский
Название основной публикации	Nucleic Acid Detection and Structural Investigations
Редакторы	Kira Astakhova, Syeda Atia Bukhari
Издатель	Humana Press Inc.
Страницы	45-56
Число страниц	12
Том	2063
ISBN (электронное издание)	978-1-0716-0138-9
ISBN (печатное издание)	978-1-0716-0137-2, 978-1-0716-0140-2
DOI	https://doi.org/10.1007/978-1-0716-0138-9_5
Состояние	Опубликовано - 1 янв 2020

Серия публикаций

Название	Methods in Molecular Biology
Том	2063
ISSN (печатное издание)	1064-3745
ISSN (электронное издание)	1940-6029

Предметные области OECD FOS+WOS

1.06.CQ БИОХИМИЯ И МОЛЕКУЛЯРНАЯ БИОЛОГИЯ
1.06.KM ГЕНЕТИКА И НАСЛЕДСТВЕННОСТЬ

Доступ к документу

10.1007/978-1-0716-0138-9_5

Другие файлы и ссылки

Link to publication in Scopus

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Цитировать

Novopashina, D. S., Semikolenova, O. A., & Venyaminova, A. G. (2020). 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. В K. Astakhova, & S. A. Bukhari (Ред.), Nucleic Acid Detection and Structural Investigations (Том 2063, стр. 45-56). (Methods in Molecular Biology; Том 2063). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0138-9_5

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abstract = "Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.",

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Novopashina, DS, Semikolenova, OA & Venyaminova, AG 2020, 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. в K Astakhova & SA Bukhari (ред.), Nucleic Acid Detection and Structural Investigations. том. 2063, Methods in Molecular Biology, том. 2063, Humana Press Inc., стр. 45-56. https://doi.org/10.1007/978-1-0716-0138-9_5

5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. / Novopashina, D. S.; Semikolenova, O. A.; Venyaminova, A. G.

Nucleic Acid Detection and Structural Investigations. ред. / Kira Astakhova; Syeda Atia Bukhari. Том 2063 Humana Press Inc., 2020. стр. 45-56 (Methods in Molecular Biology; Том 2063).

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › рецензирование

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PY - 2020/1/1

Y1 - 2020/1/1

N2 - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

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Novopashina DS, Semikolenova OA, Venyaminova AG. 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. В Astakhova K, Bukhari SA, редакторы, Nucleic Acid Detection and Structural Investigations. Том 2063. Humana Press Inc. 2020. стр. 45-56. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-0138-9_5