5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

D. S. Novopashina; O. A. Semikolenova; A. G. Venyaminova

doi:10.1007/978-1-0716-0138-9_5

5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

D. S. Novopashina, O. A. Semikolenova, A. G. Venyaminova

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › рецензирование

Аннотация

Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

Язык оригинала	английский
Название основной публикации	Methods in Molecular Biology
Издатель	Humana Press Inc.
Страницы	45-56
Число страниц	12
Том	2063
DOI	https://doi.org/10.1007/978-1-0716-0138-9_5
Состояние	Опубликовано - 1 янв 2020

Серия публикаций

Название	Methods in Molecular Biology
Том	2063
ISSN (печатное издание)	1064-3745
ISSN (электронное издание)	1940-6029

Доступ к документу

10.1007/978-1-0716-0138-9_5

Link to publication in Scopus

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Цитировать

Novopashina, D. S., Semikolenova, O. A., & Venyaminova, A. G. (2020). 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. В Methods in Molecular Biology (Том 2063, стр. 45-56). (Methods in Molecular Biology; Том 2063). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0138-9_5

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5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. / Novopashina, D. S.; Semikolenova, O. A.; Venyaminova, A. G.

Methods in Molecular Biology. Том 2063 Humana Press Inc., 2020. стр. 45-56 (Methods in Molecular Biology; Том 2063).

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › рецензирование

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