@article{8a08089ecac54a9a916a830011cb2bae,
title = "3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox",
abstract = "The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.",
keywords = "Arabidopsis, Cell cycle, Cortex, Endodermis, Epidermis, Image analysis, Pericycle, Proliferation, Protophloem, Protoxylem, RAM",
author = "Lavrekha, {Viktoriya V.} and Taras Pasternak and Klaus Palme and Mironova, {Victoria V.}",
note = "Funding Information: We thank Roland Nitschke and the staff of the Life Imaging Center (LIC) in the Center for Biological Systems Analysis (ZBSA) of the Albert-Ludwigs-University Freiburg for help with their confocal microscopy resources. This work was supported by the Russian State Budget Project (0324-2019-0040), by the Russian Federation President Grant for young scientists (MK-1297.2017.4), and by the Russian Foundation for Basic Research (RFBR-18-34-00485). This work was also supported by Bundesministerium f?r Bildung und Forschung (BMBF Microsystems, Haploswitch), theGerman Research Foundation (DFGSFB746, INST 39/839,840,841), and Deutsches Zentrum f?r Luft und Raum-fahrt (DLR 50WB1022). The authors declare that no competing interests exist. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2020",
month = jan,
day = "1",
doi = "10.1007/978-1-0716-0183-9_13",
language = "English",
volume = "2094",
pages = "119--125",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1940-6029",
}