Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016

M. Yu Kartashov; T. P. Mikryukova; N. S. Moskvitina; E. I. Krivosheina; A. I. Kuznetsov; V. N. Romanenko; N. P. Bol'shakova; V. A. Ternovoi; V. B. Loktev

doi:10.20538/1682-0363-2019-2-89-98

Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016

Переведенное название: Обнаружение и генотипирование Anaplasma phagocytophilum в клещах I. persulcatus и D. reticulatus, собранных в г. Томске в 2015-2016 гг.

M. Yu Kartashov, T. P. Mikryukova, N. S. Moskvitina, E. I. Krivosheina, A. I. Kuznetsov, V. N. Romanenko, N. P. Bol'shakova, V. A. Ternovoi, V. B. Loktev

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

Аннотация

Введение. Выявление первых случаев заболевания клещевым гранулоцитарным анаплазмозом человека в Российской Федерации, обнаружение генетических маркеров анаплазмозов в иксодовых клещах, регистрация значительного количества случаев различных клещевых инфекций на юге Западной Сибири ставят вопрос о возможной циркуляции возбудителя анаплазмоза в г. Томске и его пригородах. Цель исследования. Изучение распространения и видового разнообразия A. phagocytophilum в иксодовых клещах на территории Томской области. Материалы и методы. Проведен анализ 690 индивидуальных образцов личинок и имаго иксодовых клещей видов Ixodes persulcatus (n = 530) и Dermacentor reticulatus (n = 160), собранных в 2015-2016 гг. на территории городских и пригородных биотопов г. Томска. Первичный скрининг клещей на наличие генетического материала A. phagocytophilum проводили с помощью двухраундовой полимеразной цепной реакции в присутствии родоспецифичных праймеров из области гена 16S рРНК. Для положительных изолятов осуществлялось амплифицирование фрагмента (1 220 пар нуклеотидов) groESL-оперона белков теплового шока с последующим определением нуклеотидной последовательности фрагмента гена и проведением филогенетического анализа. Результаты. Уровень инфицированности A. phagocytophilum у личинок I. persulcatus составил (1,2 ± 0,6)%; у половозрелых особей I. persulcatus - (1,8 ± 0,7)%; у половозрелых особей D. reticulatus-(0,6 ± 0,3)%. Анализ нуклеотидной последовательности фрагмента groESL-оперона для девяти изолятов подтвердил обнаружение генетического материала возбудителя гранулоцитарного анаплазмоза. Филогенетический анализ показал, что все изоляты относятся к первой группе «нового» кластера A. phagocytophilum. Вывод. Возбудитель гранулоцитарного анаплазмоза человека впервые обнаружен в клещах I. рersulcatus, собранных в городских и пригородных биотопах г. Томска, и D. reticulatus из городского биотопа

Переведенное название	Обнаружение и генотипирование Anaplasma phagocytophilum в клещах I. persulcatus и D. reticulatus, собранных в г. Томске в 2015-2016 гг.
Язык оригинала	английский
Страницы (с-по)	89-98
Число страниц	10
Журнал	Bulletin of Siberian Medicine
Том	18
Номер выпуска	2
DOI	https://doi.org/10.20538/1682-0363-2019-2-89-98
Состояние	Опубликовано - 1 янв 2019

Ключевые слова

Anaplasma phagocytophilum
Dermacentor reticulatus
Human granulocytic anaplasmosis
Ixodes persulcatus

ГРНТИ

34.25 Вирусология

Доступ к документу

10.20538/1682-0363-2019-2-89-98

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Kartashov, M. Y., Mikryukova, T. P., Moskvitina, N. S., Krivosheina, E. I., Kuznetsov, A. I., Romanenko, V. N., Bol'shakova, N. P., Ternovoi, V. A., & Loktev, V. B. (2019). Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016. Bulletin of Siberian Medicine, 18(2), 89-98. https://doi.org/10.20538/1682-0363-2019-2-89-98

Kartashov, M. Yu ; Mikryukova, T. P. ; Moskvitina, N. S. ; Krivosheina, E. I. ; Kuznetsov, A. I. ; Romanenko, V. N. ; Bol'shakova, N. P. ; Ternovoi, V. A. ; Loktev, V. B. / Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016. В: Bulletin of Siberian Medicine. 2019 ; Том 18, № 2. стр. 89-98.

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title = "Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016",

abstract = "Introduction. The detection of the first cases of tick-borne human granulocytic anaplasmosis in Russia, discovery of genetic markers for Anaplasma spp. in ixodid ticks and reporting of a significant number of cases of tick-borne infections in the southern part of Western Siberia give reason to suppose that causative agents of tick-borne anaplasmosis may be transmitted in Tomsk and its suburbs. Objective. To study the distribution and species biodiversity of A. phagocytophilum in ixodid ticks in Tomsk Region. Materials and methods. The analysis of 690 individual ixodid ticks (larvae and adults) was carried out for Ixodes persulcatus (n = 530) and Dermacentor reticulatus (n = 160) ticks collected in 2015-2016 on the territory of urban and suburban biotopes of Tomsk. Primary screening of ticks for the presence of genetic material of A. phagocytophilum was conducted using two-round PCR with species-specific primers for the 16S rRNA gene. The amplification (1,220 kB) of the groESL fragment of the heat shock protein operon was performed for positive isolates with subsequent determination of the nucleotide sequence in the gene fragment for phylogenetic analysis. Results. The number of A. phagocytophilum positive samples for I. persulcatus (larvae) was 1.2 ± 0.6%, I. persulcatus (adult) was 1.8 ± 0.7%; and D. reticulatus (adult) was 0.6 ± 0.3%. Analysis of the nucleotide sequence of the gene fragments in groESL operon for nine isolates confirmed that the genetic material of the granulocytic anaplasmosis was detected. Phylogenetic analysis showed that all the isolates belonged to the first group of the “new cluster” of A. phagocytophilum. Conclusion. The causative agent of human granulocytic anaplasmosis has been newly detected in I. persulcatus ticks collected in urban and suburban biotopes of Tomsk and in D. reticulatus from urban foci.",

keywords = "Anaplasma phagocytophilum, Dermacentor reticulatus, Human granulocytic anaplasmosis, Ixodes persulcatus, human granulocytic anaplasmosis, Anaplasma phagocytophilum, Ixodes persulcatus, Dermacentor reticulatus, SMALL MAMMALS, PATHOGENS, OPERON",

author = "Kartashov, {M. Yu} and Mikryukova, {T. P.} and Moskvitina, {N. S.} and Krivosheina, {E. I.} and Kuznetsov, {A. I.} and Romanenko, {V. N.} and Bol'shakova, {N. P.} and Ternovoi, {V. A.} and Loktev, {V. B.}",

year = "2019",

month = jan,

day = "1",

doi = "10.20538/1682-0363-2019-2-89-98",

language = "English",

volume = "18",

pages = "89--98",

journal = "Bulletin of Siberian Medicine",

issn = "1682-0363",

publisher = "Siberian State Medical University",

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Kartashov, MY, Mikryukova, TP, Moskvitina, NS, Krivosheina, EI, Kuznetsov, AI, Romanenko, VN, Bol'shakova, NP, Ternovoi, VA & Loktev, VB 2019, 'Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016', Bulletin of Siberian Medicine, том. 18, № 2, стр. 89-98. https://doi.org/10.20538/1682-0363-2019-2-89-98

Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016. / Kartashov, M. Yu; Mikryukova, T. P.; Moskvitina, N. S.; Krivosheina, E. I.; Kuznetsov, A. I.; Romanenko, V. N.; Bol'shakova, N. P.; Ternovoi, V. A.; Loktev, V. B.

В: Bulletin of Siberian Medicine, Том 18, № 2, 01.01.2019, стр. 89-98.

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

TY - JOUR

T1 - Detection and genotyping of Anaplasma phagocytophilum in I. Persulcatus and D. Reticulatus ticks collected in Tomsk (Western Siberia) in 2015-2016

AU - Kartashov, M. Yu

AU - Mikryukova, T. P.

AU - Moskvitina, N. S.

AU - Krivosheina, E. I.

AU - Kuznetsov, A. I.

AU - Romanenko, V. N.

AU - Bol'shakova, N. P.

AU - Ternovoi, V. A.

AU - Loktev, V. B.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Introduction. The detection of the first cases of tick-borne human granulocytic anaplasmosis in Russia, discovery of genetic markers for Anaplasma spp. in ixodid ticks and reporting of a significant number of cases of tick-borne infections in the southern part of Western Siberia give reason to suppose that causative agents of tick-borne anaplasmosis may be transmitted in Tomsk and its suburbs. Objective. To study the distribution and species biodiversity of A. phagocytophilum in ixodid ticks in Tomsk Region. Materials and methods. The analysis of 690 individual ixodid ticks (larvae and adults) was carried out for Ixodes persulcatus (n = 530) and Dermacentor reticulatus (n = 160) ticks collected in 2015-2016 on the territory of urban and suburban biotopes of Tomsk. Primary screening of ticks for the presence of genetic material of A. phagocytophilum was conducted using two-round PCR with species-specific primers for the 16S rRNA gene. The amplification (1,220 kB) of the groESL fragment of the heat shock protein operon was performed for positive isolates with subsequent determination of the nucleotide sequence in the gene fragment for phylogenetic analysis. Results. The number of A. phagocytophilum positive samples for I. persulcatus (larvae) was 1.2 ± 0.6%, I. persulcatus (adult) was 1.8 ± 0.7%; and D. reticulatus (adult) was 0.6 ± 0.3%. Analysis of the nucleotide sequence of the gene fragments in groESL operon for nine isolates confirmed that the genetic material of the granulocytic anaplasmosis was detected. Phylogenetic analysis showed that all the isolates belonged to the first group of the “new cluster” of A. phagocytophilum. Conclusion. The causative agent of human granulocytic anaplasmosis has been newly detected in I. persulcatus ticks collected in urban and suburban biotopes of Tomsk and in D. reticulatus from urban foci.

AB - Introduction. The detection of the first cases of tick-borne human granulocytic anaplasmosis in Russia, discovery of genetic markers for Anaplasma spp. in ixodid ticks and reporting of a significant number of cases of tick-borne infections in the southern part of Western Siberia give reason to suppose that causative agents of tick-borne anaplasmosis may be transmitted in Tomsk and its suburbs. Objective. To study the distribution and species biodiversity of A. phagocytophilum in ixodid ticks in Tomsk Region. Materials and methods. The analysis of 690 individual ixodid ticks (larvae and adults) was carried out for Ixodes persulcatus (n = 530) and Dermacentor reticulatus (n = 160) ticks collected in 2015-2016 on the territory of urban and suburban biotopes of Tomsk. Primary screening of ticks for the presence of genetic material of A. phagocytophilum was conducted using two-round PCR with species-specific primers for the 16S rRNA gene. The amplification (1,220 kB) of the groESL fragment of the heat shock protein operon was performed for positive isolates with subsequent determination of the nucleotide sequence in the gene fragment for phylogenetic analysis. Results. The number of A. phagocytophilum positive samples for I. persulcatus (larvae) was 1.2 ± 0.6%, I. persulcatus (adult) was 1.8 ± 0.7%; and D. reticulatus (adult) was 0.6 ± 0.3%. Analysis of the nucleotide sequence of the gene fragments in groESL operon for nine isolates confirmed that the genetic material of the granulocytic anaplasmosis was detected. Phylogenetic analysis showed that all the isolates belonged to the first group of the “new cluster” of A. phagocytophilum. Conclusion. The causative agent of human granulocytic anaplasmosis has been newly detected in I. persulcatus ticks collected in urban and suburban biotopes of Tomsk and in D. reticulatus from urban foci.

KW - Anaplasma phagocytophilum

KW - Dermacentor reticulatus

KW - Human granulocytic anaplasmosis

KW - Ixodes persulcatus

KW - human granulocytic anaplasmosis

KW - Anaplasma phagocytophilum

KW - Ixodes persulcatus

KW - Dermacentor reticulatus

KW - SMALL MAMMALS

KW - PATHOGENS

KW - OPERON

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