Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich; N. V. Yunusova; M. N. Stakheeva; A. K. Somov; A. Y. Frolova; N. A. Kirushina; S. G. Afanasyev; A. E. Grigoryeva; P. P. Laktionov; I. V. Kondakova

doi:10.18097/PBMC20176302165

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich, N. V. Yunusova, M. N. Stakheeva, A. K. Somov, A. Y. Frolova, N. A. Kirushina, S. G. Afanasyev, A. E. Grigoryeva, P. P. Laktionov, I. V. Kondakova

Section of Molecular Biology and Biotechnology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.

Translated title of the contribution	Vydelenie i kharakterizatsiia ékzosom plazmy krovi bol'nykh rakom molochnoĭ zhelezy i kolorektal'nym rakom
Original language	English
Pages (from-to)	165-169
Number of pages	5
Journal	Biomeditsinskaya Khimiya
Volume	63
Issue number	2
DOIs	https://doi.org/10.18097/PBMC20176302165
Publication status	Published - Mar 2017

Keywords

Breast cancer
Colorectal cancer
Exosomes
Flow cytometry
Transmission electron microscopy

Access to Document

10.18097/PBMC20176302165

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients'. Together they form a unique fingerprint.

Cite this

Tamkovich, S. N., Yunusova, N. V., Stakheeva, M. N., Somov, A. K., Frolova, A. Y., Kirushina, N. A., Afanasyev, S. G., Grigoryeva, A. E., Laktionov, P. P., & Kondakova, I. V. (2017). Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. Biomeditsinskaya Khimiya, 63(2), 165-169. https://doi.org/10.18097/PBMC20176302165

@article{d2e6ea46c10447e7b55929cb344f5fc8,

title = "Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients",

abstract = "A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.",

keywords = "Breast cancer, Colorectal cancer, Exosomes, Flow cytometry, Transmission electron microscopy, Biomarkers/blood, Breast Neoplasms/blood, CD24 Antigen/blood, Case-Control Studies, Colorectal Neoplasms/blood, Exosomes/chemistry, Female, Gene Expression, Humans, Male, Middle Aged, Tetraspanin 28/blood, Tetraspanin 29/blood",

author = "Tamkovich, {S. N.} and Yunusova, {N. V.} and Stakheeva, {M. N.} and Somov, {A. K.} and Frolova, {A. Y.} and Kirushina, {N. A.} and Afanasyev, {S. G.} and Grigoryeva, {A. E.} and Laktionov, {P. P.} and Kondakova, {I. V.}",

year = "2017",

month = mar,

doi = "10.18097/PBMC20176302165",

language = "English",

volume = "63",

pages = "165--169",

journal = "Biomeditsinskaya Khimiya",

issn = "2310-6905",

publisher = "Rossiiskaya Akademiya Meditsinskikh Nauk",

number = "2",

}

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. / Tamkovich, S. N.; Yunusova, N. V.; Stakheeva, M. N.; Somov, A. K.; Frolova, A. Y.; Kirushina, N. A.; Afanasyev, S. G.; Grigoryeva, A. E.; Laktionov, P. P.; Kondakova, I. V.

In: Biomeditsinskaya Khimiya, Vol. 63, No. 2, 03.2017, p. 165-169.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

AU - Tamkovich, S. N.

AU - Yunusova, N. V.

AU - Stakheeva, M. N.

AU - Somov, A. K.

AU - Frolova, A. Y.

AU - Kirushina, N. A.

AU - Afanasyev, S. G.

AU - Grigoryeva, A. E.

AU - Laktionov, P. P.

AU - Kondakova, I. V.

PY - 2017/3

Y1 - 2017/3

N2 - A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.

AB - A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.

KW - Breast cancer

KW - Colorectal cancer

KW - Exosomes

KW - Flow cytometry

KW - Transmission electron microscopy

KW - Biomarkers/blood

KW - Breast Neoplasms/blood

KW - CD24 Antigen/blood

KW - Case-Control Studies

KW - Colorectal Neoplasms/blood

KW - Exosomes/chemistry

KW - Female

KW - Gene Expression

KW - Humans

KW - Male

KW - Middle Aged

KW - Tetraspanin 28/blood

KW - Tetraspanin 29/blood

UR - http://www.scopus.com/inward/record.url?scp=85027036050&partnerID=8YFLogxK

U2 - 10.18097/PBMC20176302165

DO - 10.18097/PBMC20176302165

M3 - Article

C2 - 28414289

AN - SCOPUS:85027036050

VL - 63

SP - 165

EP - 169

JO - Biomeditsinskaya Khimiya

JF - Biomeditsinskaya Khimiya

SN - 2310-6905

IS - 2

ER -