Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich, N. V. Yunusova, M. N. Stakheeva, A. K. Somov, A. Y. Frolova, N. A. Kirushina, S. G. Afanasyev, A. E. Grigoryeva, P. P. Laktionov, I. V. Kondakova

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.

Translated title of the contributionVydelenie i kharakterizatsiia ékzosom plazmy krovi bol'nykh rakom molochnoĭ zhelezy i kolorektal'nym rakom
Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalBiomeditsinskaya Khimiya
Volume63
Issue number2
DOIs
Publication statusPublished - Mar 2017

Keywords

  • Breast cancer
  • Colorectal cancer
  • Exosomes
  • Flow cytometry
  • Transmission electron microscopy

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