Trityl radicals as spin labels

Olesya Krumkacheva, Elena Bagryanskaya

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

12 Citations (Scopus)

Abstract

During the last 30 years, tetrathiatriarylmethyl (TAM) radicals have been widely used as spin probes for oxymetry in electron paramagnetic resonance (EPR) spectroscopy and EPR tomography because of the narrow EPR linewidth and high stability in living systems. Recently, some researchers proposed to use TAMs as spin labels for studies on the structure of proteins and nucleic acids using site-directed spin labeling (SDSL) and pulsed dipolar EPR spectroscopy. In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. Examples of applications of TAMs to research on the structure and functions of biopolymers are presented from recently published papers.

Original languageEnglish
Title of host publicationElectron Paramagnetic ResonanceL
Subtitle of host publicationVolume 25
Editors Chechik, DM Murphy
PublisherRoyal Society of Chemistry
Pages35-60
Number of pages26
ISBN (Electronic)9781782628576
ISBN (Print)978-1-78262-857-6
DOIs
Publication statusPublished - 1 Jan 2017

Publication series

NameElectron Paramagnetic Resonance
Volume25
ISSN (Print)1464-4622
ISSN (Electronic)1465-1955

Keywords

  • ELECTRON-PARAMAGNETIC-RESONANCE
  • INTRACELLULAR OXYGEN PROBES
  • PULSED DIPOLAR EPR
  • DISTANCE MEASUREMENTS
  • TRIARYLMETHYL RADICALS
  • ROOM-TEMPERATURE
  • PHYSIOLOGICAL TEMPERATURES
  • RELAXATION-ENHANCEMENT
  • MAGNETIC-RESONANCE
  • TISSUE OXYGEN

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