Transient protein–protein complexes in base excision repair

Anton V. Endutkin, Anna V. Yudkina, Viktoriya S. Sidorenko, Dmitry O. Zharkov

Research output: Contribution to journalReview articlepeer-review

4 Citations (Scopus)

Abstract

Transient protein–protein complexes are of great importance for organizing multiple enzymatic reactions into productive reaction pathways. Base excision repair (BER), a process of critical importance for maintaining genome stability against a plethora of DNA-damaging factors, involves several enzymes, including DNA glycosylases, AP endonucleases, DNA polymerases, DNA ligases and accessory proteins acting sequentially on the same damaged site in DNA. Rather than being assembled into one stable multisubunit complex, these enzymes pass the repair intermediates between them in a highly coordinated manner. In this review, we discuss the nature and the role of transient complexes arising during BER as deduced from structural and kinetic data. Almost all of the transient complexes are DNA-mediated, although some may also exist in solution and strengthen under specific conditions. The best-studied example, the interactions between DNA glycosylases and AP endonucleases, is discussed in more detail to provide a framework for distinguishing between stable and transient complexes based on the kinetic data.

Original languageEnglish
Pages (from-to)4407-4418
Number of pages12
JournalJournal of Biomolecular Structure and Dynamics
Volume37
Issue number17
DOIs
Publication statusPublished - 22 Nov 2019

Keywords

  • AP endonucleases
  • Base excision repair
  • DNA glycosylases
  • transient protein–protein complexes

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