Towards optical control of single blood platelet activation

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Abstract

Blood platelets play a pivotal role in blood coagulation and in other normal and pathological processes. The understanding of fundamental mechanisms underlying their functions is very important for diagnostics and treatment. Single-cell experiments are needed for this purpose, which are complicated by insufficient spatiotemporal precision of conventional activation protocols. We present an approach to trigger single platelet activation optically, without the need of reagent mixing. This is achieved using photolabile compound, which rapidly delivers epinephrine upon UV irradiation. We demonstrated the applicability of the technique to rapidly induce platelet activation for studying dynamics of activation. The presented method may give novel fundamental knowledge about platelet functions and facilitate current research of their ability to deliver drugs to tumors or vascular injury sites.

Original languageEnglish
Title of host publicationSARATOV FALL MEETING 2017: LASER PHYSICS AND PHOTONICS XVIII; AND COMPUTATIONAL BIOPHYSICS AND ANALYSIS OF BIOMEDICAL DATA IV
Subtitle of host publicationLaser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV
EditorsVL Derbov, DE Postnov
PublisherSPIE
Number of pages7
Volume10717
ISBN (Electronic)9781510620032
DOIs
Publication statusPublished - 1 Jan 2018
EventSaratov Fall Meeting 2017: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV - Saratov, Russian Federation
Duration: 26 Sep 201730 Sep 2017

Publication series

NameProceedings of SPIE
PublisherSPIE-INT SOC OPTICAL ENGINEERING
Volume10717
ISSN (Print)0277-786X

Conference

ConferenceSaratov Fall Meeting 2017: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV
CountryRussian Federation
CitySaratov
Period26.09.201730.09.2017

Keywords

  • Biophotonics
  • Caged epinephrine
  • Platelet activation
  • Single cell optical control
  • caged epinephrine
  • biophotonics
  • P2Y(1)
  • SHAPE CHANGE
  • single cell optical control
  • MODEL
  • platelet activation

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  • Cite this

    Spiryova, D. V., Karmatskih, O. Y., Vorob'Ev, A. Y., & Moskalensky, A. E. (2018). Towards optical control of single blood platelet activation. In VL. Derbov, & DE. Postnov (Eds.), SARATOV FALL MEETING 2017: LASER PHYSICS AND PHOTONICS XVIII; AND COMPUTATIONAL BIOPHYSICS AND ANALYSIS OF BIOMEDICAL DATA IV: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV (Vol. 10717). [1071722] (Proceedings of SPIE; Vol. 10717). SPIE. https://doi.org/10.1117/12.2305477