The Effect of the Melatonin-containing Drug on the Histological Structure of the Organs of the Immune System of Mice under Round-the-Clock Illumination

A. V. Shurlygina, A. E. Serykh, S. V. Michurina, E. E. Rachkovsky

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

In modern-day conditions, the prevalence of situations in which light disrupts one's daily life has increased. This causes a decrease in the synthesis of melatonin at nighttime. Melatonin affects the body's lymphocytes differentiation, inflammation and immune responses. Therefore it is important to study the effect of light disturbance on the organs of the immune system as well as the possibilities of correcting immunity disorders with the help of a new melatonin-containing drug (Complex M), synthesized in the laboratory of pharmaceutical technologies of Research Institute of Clinical and Experimental Lymphology (RICEL), a branch of the Institute of Cytology and Genetics (ICG). The purpose of this study is to identify the morphological features of the thymus and spleen of mice after intragastric administration of the Complex M within 7 days alongside round-the-clock illumination. The experiment used male C57Bl/6J SPF mice aged 10-12 weeks. The day after the last administration of Complex M, the animals were sacrificed by cranio-cervical dislocation and the thymus and spleen samples were taken. The spleen and thymus samples were fixed by immersion in 10% buffered formalin and enclosed in Histomix. Sections were stained with hematoxylin and eosin and morphometric analysis was performed using ImagePro and Image J programms. The administration of Complex M led to an increase in the diameter of the pulp veins and an increase in the cortico-medullary index of the thymus, which indicates its activating effect on the central differentiation of T-lymphocytes.

Original languageEnglish
Title of host publicationProceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages245-249
Number of pages5
ISBN (Electronic)9781728195971
DOIs
Publication statusPublished - Jul 2020
Event2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020 - Novosibirsk, Russian Federation
Duration: 6 Jul 202010 Jul 2020

Publication series

NameProceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

Conference

Conference2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
CountryRussian Federation
CityNovosibirsk
Period06.07.202010.07.2020

Keywords

  • Complex M
  • melatonin
  • polydimethylsiloxane
  • sorbent
  • spleen
  • thymus

OECD FOS+WOS

  • 1.02 COMPUTER AND INFORMATION SCIENCES
  • 1.06 BIOLOGICAL SCIENCES
  • 3.01 BASIC MEDICAL RESEARCH
  • 3.03 HEALTH SCIENCES
  • 5.09 OTHER SOCIAL SCIENCES

State classification of scientific and technological information

  • 76.03 Biomedical discipline

Fingerprint

Dive into the research topics of 'The Effect of the Melatonin-containing Drug on the Histological Structure of the Organs of the Immune System of Mice under Round-the-Clock Illumination'. Together they form a unique fingerprint.

Cite this