The development of a liquid chromatography high‐resolution mass spectrometric method for apixaban quantification in dried plasma spots in parallel reaction monitoring mode

Alexander Chernonosov, Liliya Aksenova, Vladimir Koval

Research output: Contribution to journalArticlepeer-review

Abstract

This work aimed at developing and validating a rapid, sensitive, and robust method of liquid chromatography with high‐resolution mass spectrometry (LC–HRMS) in parallel reaction monitoring (PRM) mode for apixaban quantification in dried plasma spots (DPSs) with a simple extraction procedure. A 25 μL sample of human plasma was placed onto Whatman 903 Protein Saver Cards and allowed to dry; 3.2 mm diameter disks were cut out from DPSs using a puncher, and 100 μL of a working internal standard solution was added to each sample. After this, they were vortexed on a shaker for 15 min at 800 rpm and 40 °C and quick centrifugation (10,000× g, 10 s), and then the extracts were transferred into a 300 μL vial for LC–HRMS. Data were acquired in PRM mode via detection of all target product ions with 10 ppm tolerance. Total analysis time was 5 min. The LC–HRMS method was validated for the 10–400 ng/mL range with R2 > 0.99. Within this range, intra‐ and interday variability of precision and accuracy was <10%, and recovery was 69.7–85.1%. Apixaban was stable after brief storage at room tem-perature, and at 4 °C for up to a month. The method development and validation results proved that this LC–HRMS assay of apixaban in DPSs is selective and robust.

Original languageEnglish
Article number450
Pages (from-to)1-12
Number of pages12
JournalProcesses
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 2021

Keywords

  • Apixaban
  • DPS
  • Dried blood spot
  • Dried plasma spot
  • High‐resolution mass spectrometry
  • LC–HRMS
  • Parallel reaction monitoring

OECD FOS+WOS

  • 2.04 CHEMICAL ENGINEERING
  • 1.04.EE CHEMISTRY, ORGANIC

Fingerprint

Dive into the research topics of 'The development of a liquid chromatography high‐resolution mass spectrometric method for apixaban quantification in dried plasma spots in parallel reaction monitoring mode'. Together they form a unique fingerprint.

Cite this