The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity

Anna Timofeeva, Sergey Sedykh, Lada Maksimenko, Tatyana Sedykh, Sergey Skudarnov, Tatyana Ostapova, Svetlana Yaschenko, Natalya Gashnikova, Georgy Nevinsky

Research output: Contribution to journalArticlepeer-review

Abstract

Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.

Original languageEnglish
Article number304
JournalLife
Volume12
Issue number2
DOIs
Publication statusPublished - Feb 2022

Keywords

  • Abzymes
  • Amylolytic activity
  • Bispecific antibodies
  • Catalytic activity
  • DNA hydrolysis
  • DNAse activity
  • ELISA
  • HIV
  • IgG
  • Immunoglobulin G

OECD FOS+WOS

  • 1.06 BIOLOGICAL SCIENCES
  • 1.06.QU MICROBIOLOGY
  • 3.03.WV SOCIAL SCIENCES, BIOMEDICAL

Fingerprint

Dive into the research topics of 'The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity'. Together they form a unique fingerprint.

Cite this