Substrate specificity of healthy human sera IgG antibodies with peroxidase and oxydoreductase activities

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Abstract

We have carried out an analysis of whether blood IgG antibodies can protect humans from oxidative stress by oxidizing different harmful compounds. A somewhat unexpected result was obtained. We show here for the first time that healthy human sera IgGs with the peroxidase (in the presence H2 O2) efficiently oxidize different compounds: 3,3 -diaminobenzidine (1; DAB), 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (2; ATBS), o-phenylenediamine (3; OPD), homovanillic acid (4; HVA), α-naphthol (5), 5-aminosalicylic acid (6; 5-ASA) and 3-amino-9-ethylcarbazole (7; AEC), but seven of nine IgG preparations from different volunteers cannot oxidize p-hydroquinone (8: pHQ). The average apparent kcat values in the H2 O2 -dependent oxidation by human IgGs decreased in the following order (min−1): ATBS (73.7) ≥ DAB (66.3) > AEC (38.0) ≥ HVA (19.8) ≥ α-naphthol (8.6) > OPD (0.62) ≥ 5-ASA (0.48) > pHQ (0.24). In the absence of H2 O2 (oxidoreductase activity), the relative average kcat values decreased in the following order (min−1): DAB (52.1) ≥ ATBS (50.5) > OPD (0.25). The peroxidase average activity of human IgGs was higher than the oxidoreductase one: 1.2-, 1.5- and 2.5-fold for DAB, ATBS and OPD, respectively. It should be assumed that antibodies can oxidize in addition to the large number of other different compounds analysed by us. As a whole, the specific wide repertoire of polyclonal human IgGs oxidizing various compounds could play an important role in protecting humans from oxidative stress and serve as an additional natural system destroying H2O2 and different toxic mutagenic and carcinogenic compounds.

Original languageEnglish
Article number171097
Pages (from-to)171097
Number of pages13
JournalRoyal Society Open Science
Volume5
Issue number1
DOIs
Publication statusPublished - 31 Jan 2018

Keywords

  • Abzymes
  • IgGs
  • Oxidoreductase and peroxidase activities
  • Sera of healthy human
  • Substrate specificities
  • AUTOANTIBODIES
  • CATALYTIC ANTIBODIES
  • sera of healthy human
  • COLONY FORMATION
  • ANTIOXIDANT ENZYMES
  • HYDROLYSIS
  • RATS
  • ABZYMES
  • oxidoreductase and peroxidase activities
  • MYELIN BASIC-PROTEIN
  • AUTOIMMUNE
  • substrate specificities
  • OXIDOREDUCTASE ACTIVITIES
  • abzymes

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