Study of calcium signaling dynamics in single platelets using optical activation methods

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Abstract

Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.

Original languageEnglish
Title of host publicationBiomedical Spectroscopy, Microscopy, and Imaging
EditorsJurgen Popp, Csilla Gergely
PublisherSPIE
ISBN (Electronic)9781510634909
DOIs
Publication statusPublished - 1 Apr 2020
EventBiomedical Spectroscopy, Microscopy, and Imaging 2020 - Virtual, Online, France
Duration: 6 Apr 202010 Apr 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11359
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceBiomedical Spectroscopy, Microscopy, and Imaging 2020
CountryFrance
CityVirtual, Online
Period06.04.202010.04.2020

Keywords

  • Activation
  • Caged ADP
  • Caged Epinephrine
  • Calcium oscillations
  • Delay time
  • Microscope
  • Platelet
  • TrackMate

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