Spectrally encoded microspheres for immunofluorescence analysis

Natalya Sankova, Pavel Shalaev, Viktoriya Semeykina, Sergey Dolgushin, Elena Odintsova, Ekaterina Parkhomchuk

Research output: Contribution to journalReview articlepeer-review

Abstract

A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis.

Original languageEnglish
Article number49890
Number of pages21
JournalJournal of Applied Polymer Science
Volume138
Issue number8
Early online date28 Sep 2020
DOIs
Publication statusPublished - 20 Feb 2021

Keywords

  • bioengineering
  • biomedical applications
  • functionalization of polymers
  • synthesis and processing techniques
  • MOLECULAR-WEIGHT
  • DISPERSION POLYMERIZATION
  • EMULSION
  • IRON-OXIDE
  • NANOPARTICLES
  • SUSPENSION
  • SEEDED POLYMERIZATION
  • MONODISPERSE POLYSTYRENE MICROSPHERES
  • REACTION PARAMETERS
  • PARTICLE-SIZE

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