Spectral solution of the inverse Mie problem

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4 Citations (Scopus)

Abstract

We developed a fast method to determine size and refractive index of homogeneous spheres from the power Fourier spectrum of their light-scattering patterns (LSPs), measured with the scanning flow cytometer. Specifically, we used two spectral parameters: the location of the non-zero peak and zero-frequency amplitude, and numerically inverted the map from the space of particle characteristics (size and refractive index) to the space of spectral parameters. The latter parameters can be reliably resolved only for particle size parameter greater than 11, and the inversion is unique only in the limited range of refractive index with upper limit between 1.1 and 1.25 (relative to the medium) depending on the size parameter and particular definition of uniqueness. The developed method was tested on two experimental samples, milk fat globules and spherized red blood cells, and resulted in accuracy not worse than the reference method based on the least-square fit of the LSP with the Mie theory. Moreover, for particles with significant deviation from the spherical shape the spectral method was much closer to the Mie-fit result than the estimated uncertainty of the latter. The spectral method also showed adequate results for synthetic LSPs of spheroids with aspect ratios up to 1.4. Overall, we present a general framework, which can be used to construct an inverse algorithm for any other experimental signals.

Original languageEnglish
Pages (from-to)280-294
Number of pages15
JournalJournal of Quantitative Spectroscopy and Radiative Transfer
Volume200
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Fourier spectrum
  • Inverse problem
  • Light scattering
  • Mie theory
  • Single-particle characterization
  • LIGHT-SCATTERING PROBLEM
  • SIZE
  • SCANNING FLOW-CYTOMETRY
  • NONSPHERICAL PARTICLES
  • PRECISION
  • SPHERICAL-PARTICLES
  • SHAPE
  • ERYTHROCYTES
  • REFRACTIVE-INDEX
  • RED-BLOOD-CELLS

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