Spatial specificity of auxin responses coordinates wood formation

Klaus Brackmann, Jiyan Qi, Michael Gebert, Virginie Jouannet, Theresa Schlamp, Karin Grünwald, Eva Sophie Wallner, Daria D. Novikova, Victor G. Levitsky, Javier Agustí, Pablo Sanchez, Jan U. Lohmann, Thomas Greb

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Spatial organization of signalling events of the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, the stem cell niche mediating wood formation, fundamentally depends on auxin signalling but its exact role and spatial organization is obscure. Here we show that, while auxin signalling levels increase in differentiating cambium descendants, a moderate level of signalling in cambial stem cells is essential for cambium activity. We identify the auxin-dependent transcription factor ARF5/MONOPTEROS to cell-autonomously restrict the number of stem cells by directly attenuating the activity of the stem cell-promoting WOX4 gene. In contrast, ARF3 and ARF4 function as cambium activators in a redundant fashion from outside of WOX4-expressing cells. Our results reveal an influence of auxin signalling on distinct cambium features by specific signalling components and allow the conceptual integration of plant stem cell systems with distinct anatomies.

Original languageEnglish
Article number875
Pages (from-to)875
Number of pages15
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 28 Feb 2018

Keywords

  • Arabidopsis Proteins/biosynthesis
  • Arabidopsis/genetics
  • Cambium/cytology
  • Cell Proliferation/physiology
  • DNA-Binding Proteins/metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant/genetics
  • Homeodomain Proteins/biosynthesis
  • Indoleacetic Acids/metabolism
  • Nuclear Proteins/metabolism
  • Plant Growth Regulators/metabolism
  • Plants, Genetically Modified/metabolism
  • Signal Transduction
  • Stem Cells/cytology
  • Transcription Factors/metabolism
  • Wood/cytology
  • ARABIDOPSIS-THALIANA
  • SHOOT
  • ROOT APICAL MERISTEM
  • FACTOR MONOPTEROS
  • VASCULAR CELL-DIVISION
  • HYBRID ASPEN
  • SECONDARY GROWTH
  • GENE FAMILY
  • PLANTS
  • TRANSCRIPTION FACTOR

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