Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae)

Tatyana V. Karamysheva, Anna A. Torgasheva, Yaroslav R. Yefremov, Anton G. Bogomolov, Thomas Liehr, Pavel M. Borodin, Nikolay B. Rubtsov, M. Puertas

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Korean field mouse (Apodemus peninsulae) shows a wide variation in the number of B chromosomes composed of constitutive heterochromatin. For this reason, it provides a good model to study the influence of the number of centromeres and amount of heterochromatin on spatial organization of interphase nuclei. We analyzed the three-dimensional organization of fibroblast and spermatocyte nuclei of the field mice carrying a different number of B chromosomes using laser scanning microscopy and 3D fluorescence in situ hybridization. We detected a co-localization of the B chromosomes with constitutive heterochromatin of the chromosomes of the basic set. We showed a non-random distribution of B chromosomes in the spermatocyte nuclei. Unpaired B chromosomes showed a tendency to occur in the compartment formed by the unpaired part of the XY bivalent.

Original languageEnglish
Pages (from-to)815-824
Number of pages10
JournalGenome
Volume60
Issue number10
DOIs
Publication statusPublished - Oct 2017

Keywords

  • A chromosomes
  • B chromosomes
  • Centromeres
  • Confocal microscopy
  • FISH on three-dimensionally preserved nuclei
  • Spermatocytes
  • Three-dimensional architecture of the nucleus
  • CELLS
  • CHROMATIN
  • three-dimensional architecture of the nucleus
  • MAMMALIAN MEIOSIS
  • CENTROMERES
  • INTERPHASE
  • GENOME
  • spermatocytes
  • confocal microscopy
  • COMPARATIVE FISH ANALYSIS
  • ARCHITECTURE
  • centromeres
  • MICE
  • MICRODISSECTION

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