Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo

V. A. Cherenko, E. A. Filipenko, T. S. Golubeva

Research output: Contribution to journalConference articlepeer-review

Abstract

RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.

Original languageEnglish
Article number022041
JournalIOP Conference Series: Earth and Environmental Science
Volume723
Issue number2
DOIs
Publication statusPublished - 12 Apr 2021
Event2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021 - Smolensk, Russian Federation
Duration: 25 Jan 2021 → …

OECD FOS+WOS

  • 1.05 EARTH AND RELATED ENVIRONMENTAL SCIENCES

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