Selection of reference genes for quantitative analysis of microRNA expression in three different types of cancer

Yuliya A. Veryaskina, Sergei E. Titov, Mikhail K. Ivanov, Pavel S. Ruzankin, Anton S. Tarasenko, Sergei P. Shevchenko, Igor B. Kovynev, Evgenij V. Stupak, Tatiana I. Pospelova, Igor F. Zhimulev

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs (miRNAs) are promising biomarkers in cancer research. Quantitative PCR (qPCR), also known as real-time PCR, is the most frequently used technique for measuring miRNA expression levels. The use of this technique, however, requires that expression data be normalized against reference genes. The problem is that a universal internal control for quantitative analysis of miRNA expression by qPCR has yet to be known. The aim of this work was to find the miRNAs with stable expression in the thyroid gland, brain and bone marrow according to NanoString nCounter miRNA quantification data. As a results, the most stably expressed miRNAs were as follows: miR-361-3p, -151a-3p and -29b-3p in the thyroid gland; miR-15a-5p, -194-5p and -532-5p in the brain; miR-140-5p, -148b-3p and -362-5p in bone marrow; and miR-423-5p, -28-5p and -532-5p, no matter what tissue type. These miRNAs represent promising reference genes for miRNA quantification by qPCR.

Original languageEnglish
Article numbere0254304
JournalPLoS ONE
Volume17
Issue number2 February
DOIs
Publication statusPublished - Feb 2022

Keywords

  • Biomarkers, Tumor/genetics
  • Bone Marrow Neoplasms/genetics
  • Brain Neoplasms/genetics
  • Case-Control Studies
  • Gene Expression Profiling/standards
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs/genetics
  • Prognosis
  • Reference Standards
  • Thyroid Neoplasms/genetics

OECD FOS+WOS

  • 1.01 MATHEMATICS
  • 1.06 BIOLOGICAL SCIENCES

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