Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing

K. A. Okotrub, S. Y. Amstislavsky, N. V. Surovtsev

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between −7 and 0 °C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume635
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • Cryopreservation
  • Glycerol
  • Lipid phase transition
  • Preimplantation mouse embryo
  • Program freezing
  • Raman spectroscopy
  • Molecular Conformation
  • Lipids/chemistry
  • Phase Transition
  • Blastocyst/chemistry
  • Cryopreservation/methods
  • Freezing
  • Animals
  • Lipid Bilayers/chemistry
  • Phosphatidylcholines/chemistry
  • Female
  • Mice
  • Spectrum Analysis, Raman/methods
  • POLYMETHYLENE CHAIN
  • H STRETCHING REGION
  • CRYOPRESERVATION
  • FROZEN YEAST-CELLS
  • OOCYTES
  • CHILLING SENSITIVITY
  • DEVELOPMENTAL COMPETENCE
  • INTRACELLULAR ICE FORMATION
  • FATTY-ACID
  • IN-VIVO

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