Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

M. L. Perepechaeva, T. A. Seredina, Y. A. Sidorova, E. N. Pivovarova, A. L. Markel, V. V. Lyakhovich, A. Y. Grishanova

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

Original languageEnglish
Pages (from-to)308-313
Number of pages6
JournalBiomedical and Environmental Sciences
Volume30
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

Keywords

  • Animals
  • Antioxidants/pharmacology
  • Aryl Hydrocarbon Hydroxylases/genetics
  • Benzo(a)pyrene/toxicity
  • Carcinogens/toxicity
  • Environmental Pollutants/toxicity
  • Gene Expression Regulation
  • Male
  • Mutagens/toxicity
  • Quercetin/pharmacology
  • RNA, Messenger/genetics
  • Rats
  • Rats, Wistar
  • RAT-LIVER
  • PROTEIN
  • TRANSCRIPTION
  • INDUCTION
  • 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
  • GENE
  • ARYL-HYDROCARBON RECEPTOR
  • AH-RECEPTOR
  • PROMOTER
  • BINDING

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