Abstract
We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).
Original language | English |
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Pages (from-to) | 308-313 |
Number of pages | 6 |
Journal | Biomedical and Environmental Sciences |
Volume | 30 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Apr 2017 |
Keywords
- Animals
- Antioxidants/pharmacology
- Aryl Hydrocarbon Hydroxylases/genetics
- Benzo(a)pyrene/toxicity
- Carcinogens/toxicity
- Environmental Pollutants/toxicity
- Gene Expression Regulation
- Male
- Mutagens/toxicity
- Quercetin/pharmacology
- RNA, Messenger/genetics
- Rats
- Rats, Wistar
- RAT-LIVER
- PROTEIN
- TRANSCRIPTION
- INDUCTION
- 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
- GENE
- ARYL-HYDROCARBON RECEPTOR
- AH-RECEPTOR
- PROMOTER
- BINDING