Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

M. L. Perepechaeva; T. A. Seredina; Y. A. Sidorova; E. N. Pivovarova; A. L. Markel; V. V. Lyakhovich; A. Y. Grishanova

doi:10.3967/bes2017.041

Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

M. L. Perepechaeva, T. A. Seredina, Y. A. Sidorova, E. N. Pivovarova, A. L. Markel, V. V. Lyakhovich, A. Y. Grishanova

Physiology Section

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

Original language	English
Pages (from-to)	308-313
Number of pages	6
Journal	Biomedical and Environmental Sciences
Volume	30
Issue number	4
DOIs	https://doi.org/10.3967/bes2017.041
Publication status	Published - 1 Apr 2017

Keywords

Animals
Antioxidants/pharmacology
Aryl Hydrocarbon Hydroxylases/genetics
Benzo(a)pyrene/toxicity
Carcinogens/toxicity
Environmental Pollutants/toxicity
Gene Expression Regulation
Male
Mutagens/toxicity
Quercetin/pharmacology
RNA, Messenger/genetics
Rats
Rats, Wistar
RAT-LIVER
PROTEIN
TRANSCRIPTION
INDUCTION
2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
GENE
ARYL-HYDROCARBON RECEPTOR
AH-RECEPTOR
PROMOTER
BINDING

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title = "Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression",

abstract = "We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).",

keywords = "Animals, Antioxidants/pharmacology, Aryl Hydrocarbon Hydroxylases/genetics, Benzo(a)pyrene/toxicity, Carcinogens/toxicity, Environmental Pollutants/toxicity, Gene Expression Regulation, Male, Mutagens/toxicity, Quercetin/pharmacology, RNA, Messenger/genetics, Rats, Rats, Wistar, RAT-LIVER, PROTEIN, TRANSCRIPTION, INDUCTION, 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN, GENE, ARYL-HYDROCARBON RECEPTOR, AH-RECEPTOR, PROMOTER, BINDING",

author = "Perepechaeva, {M. L.} and Seredina, {T. A.} and Sidorova, {Y. A.} and Pivovarova, {E. N.} and Markel, {A. L.} and Lyakhovich, {V. V.} and Grishanova, {A. Y.}",

year = "2017",

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doi = "10.3967/bes2017.041",

language = "English",

volume = "30",

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AU - Perepechaeva, M. L.

AU - Seredina, T. A.

AU - Sidorova, Y. A.

AU - Pivovarova, E. N.

AU - Markel, A. L.

AU - Lyakhovich, V. V.

AU - Grishanova, A. Y.

PY - 2017/4/1

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N2 - We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

AB - We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

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KW - Environmental Pollutants/toxicity

KW - Gene Expression Regulation

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