Proposed Dynamics of CDB3 Activation in Human Erythrocytes by Nifedipine Studied with Scanning Flow Cytometry

Ekaterina S. Yastrebova, Anastasiya I. Konokhova, Dmitry I. Strokotov, Andrei A. Karpenko, Valeri P. Maltsev, Andrei V. Chernyshev

Research output: Contribution to journalArticlepeer-review

Abstract

Nifedipine is calcium channels and pumps blocker widely used in medicine. However, mechanisms of nifedipine action in blood are not clear. In particular, the influence of nifedipine on erythrocytes is far from completely understood. In this work, applying scanning flow cytometry, we observed experimentally for the first time the dynamics behind a significant increase of HCO3 /Cl transmembrane exchange rate of CDB3 (main anion exchanger, AE1, Band 3, SLC4A1) of human erythrocytes in the presence of nifedipine in blood. It was found that the rate of CDB3 activation is not limited by the rate of nifedipine binding and/or Ca2+ transport. In order to explain the experimental data, we suggested a kinetic model assuming that the rate of CDB3 activation is limited by the dynamics of the balance between two intracellular processes (1) the activation of CDB3 limited by its interaction with intracellular Ca2+, and (2) the spontaneous deactivation of CDB3. Thus the use of scanning flow cytometry allowed to clarify quantitatively the molecular kinetic mechanism of nifedipine action on human erythrocytes. In particular, the efficiency (~30) and rates of activation (~0.3 min−1) and deactivation (~10−3 min−1) of CDB3 in human erythrocytes was evaluated for two donors.

Original languageEnglish
Pages (from-to)1275-1284
Number of pages10
JournalCytometry Part A
Volume95
Issue number12
DOIs
Publication statusPublished - Dec 2019

Keywords

  • adalat
  • band 3
  • calcium ions
  • kinetic modeling
  • red blood cells
  • MEMBRANES
  • CALCIUM-CHANNEL BLOCKERS
  • TRANSPORT
  • KINETICS
  • DRUGS
  • RED-BLOOD-CELLS
  • BINDING-SITES
  • EXCHANGE
  • ANTAGONISTS
  • CHLORIDE

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