Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization

Rustem R. Zairov, Anastasiya O. Solovieva, Nataliya A. Shamsutdinova, Sergey N. Podyachev, Michael A. Shestopalov, Tatiana N. Pozmogova, Svetlana M. Miroshnichenko, Asiya R. Mustafina, Andrey A. Karasik

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.

Original languageEnglish
Pages (from-to)166-173
Number of pages8
JournalMaterials Science and Engineering C
Volume95
DOIs
Publication statusPublished - 1 Feb 2019

Keywords

  • Cell label
  • Cellular uptake
  • Layer-by-layer
  • Polyelectrolyte
  • Tb(III) chelate nanoparticles
  • Microscopy, Electron, Transmission
  • Humans
  • Luminescence
  • Hep G2 Cells
  • Microscopy, Confocal
  • Flow Cytometry
  • Nanoparticles/chemistry
  • Cell Line, Tumor
  • Polyelectrolytes/chemistry

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