Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells

Artem A. Ilyin, Sergei S. Ryazansky, Semen A. Doronin, Oxana M. Olenkina, Elena A. Mikhaleva, Evgeny Y. Yakushev, Yuri A. Abramov, Stepan N. Belyakin, Anton V. Ivankin, Alexey V. Pindyurin, Vladimir A. Gvozdev, Mikhail S. Klenov, Yuri Y. Shevelyov

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwimust scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Herewe report the DamIDseq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the proteincoding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.

Original languageEnglish
Pages (from-to)7666-7680
Number of pages15
JournalNucleic Acids Research
Volume45
Issue number13
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • Animals
  • Animals, Genetically Modified
  • Argonaute Proteins/chemistry
  • Chromatin/genetics
  • DNA Transposable Elements
  • Drosophila Proteins/chemistry
  • Drosophila melanogaster/genetics
  • Female
  • Gene Silencing
  • Genome, Insect
  • Genomic Instability
  • Models, Biological
  • Nuclear Pore/genetics
  • Ovary/cytology
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • RNA, Messenger/genetics
  • PROTEIN
  • GERM-LINE
  • GENE-EXPRESSION
  • STEM-CELLS
  • THO COMPLEX
  • MESSENGER-RNA EXPORT
  • GENOME
  • PRIMARY PIRNA BIOGENESIS
  • SEQUENCING DATA
  • MELANOGASTER

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