Phosphorylation of Pnut in the early stages of Drosophila embryo development affects association of the septin complex with the membrane and is important for viability

Katarina Akhmetova, Maxim Balasov, Anton Svitin, Elena Chesnokova, Matthew Renfrow, Igor Chesnokov

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Septin proteins are polymerizing GTPases that are found in most eukaryotic species. Septins are important for cytokinesis and participate in many processes involving spatial modifications of the cell cortex. In Drosophila, septin proteins Pnut, Sep1, and Sep2 form a hexameric septin complex. Here, we found that septin protein Pnut is phosphorylated during the first 2 hr of Drosophila embryo development. To study the effect of Pnut phosphorylation in a live organism, we created a new Drosophila pnut null mutant that allows for the analysis of Pnut mutations during embryogenesis. To understand the functional significance of Pnut phosphorylation, Drosophila strains carrying nonphosphorylatable and phosphomimetic mutant pnut transgenes were established. The expression of the nonphosphorylatable Pnut protein resulted in semilethality and abnormal protein localization, whereas the expression of the phospho-mimetic mutant form of Pnut disrupted the assembly of a functional septin complex and septin filament formation in vitro. Overall, our findings indicate that the controlled phosphorylation of Pnut plays an important role in regulating septin complex functions during organism development.

Original languageEnglish
Pages (from-to)27-38
Number of pages12
JournalG3: Genes, Genomes, Genetics
Volume8
Issue number1
DOIs
Publication statusPublished - 4 Jan 2018

Keywords

  • Drosophila
  • Orc6
  • Phosphorylation
  • Pnut
  • Septins
  • Amino Acid Sequence
  • Cell Line
  • Drosophila melanogaster/cytology
  • Protein Multimerization
  • Cytoplasm/metabolism
  • Septins/genetics
  • Cytokinesis
  • Macrophages/cytology
  • Microfilament Proteins/deficiency
  • Drosophila Proteins/deficiency
  • Animals
  • Gene Expression Regulation, Developmental
  • Cell Membrane/metabolism
  • Embryo, Nonmammalian
  • Cytoskeleton/metabolism
  • Protein Binding
  • Mutation

Fingerprint Dive into the research topics of 'Phosphorylation of Pnut in the early stages of Drosophila embryo development affects association of the septin complex with the membrane and is important for viability'. Together they form a unique fingerprint.

Cite this