Peptides on the Surface: Spin-Label EPR and PELDOR Study of Adsorption of the Antimicrobial Peptides Trichogin GA IV and Ampullosporin A on the Silica Nanoparticles

Victoria N. Syryamina, Rimma I. Samoilova, Yuri D. Tsvetkov, Arkady V. Ischenko, Marta De Zotti, Marina Gobbo, Claudio Toniolo, Fernando Formaggio, Sergei A. Dzuba

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The properties of antimicrobial peptides adsorbed on inorganic or organic surfaces are of interest for their potential applications in intracellular drug delivery. In this work, continuous-wave (CW) electron paramagnetic resonance (EPR) and pulsed electron-electron double resonance (PELDOR) techniques were applied to study adsorption of the short-sequence trichogin GA IV and the medium-length sequence ampullosporin A antimicrobial peptides on the monodisperse colloidal silica nanospheres of 20 nm diameter. The results obtained by CW EPR support the view that the adsorbed peptides form close-packed clusters. PELDOR data show that both trichogin and ampullosporin adsorbed on the silica surface possess a more disordered conformation as compared to that in solution. For ampullosporin, disordering is much more pronounced than for trichogin. After desorption, the peptides restored their conformations; upon adsorption the peptides in some cases may lose partly their biradical character.

Original languageEnglish
Pages (from-to)309-320
Number of pages12
JournalApplied Magnetic Resonance
Volume47
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016

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