Pathology of A(H5N8) (Clade Virus in Experimentally Infected Chickens and Mice

Elena A. Prokopyeva, Vsevolod A. Zinserling, You Chan Bae, Yongkuk Kwon, Olga G. Kurskaya, Ivan A. Sobolev, Peter M. Kozhin, Andrey Komissarov, Artem Fadeev, Vladimir Petrov, Alexander M. Shestopalov, Kirill A. Sharshov

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6 Citations (Scopus)


The emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade virus that was isolated from sick domestic duck at commercial farm during the second wave of spread that began in October and affected poultry (ducks; chickens) in several European regions of Russia and Western Siberia in 2016. The strain was highly lethal in experimental infection of chickens and mice with IVPI = 2.34 and MLD50 = 1.3log10 = EID50, accordingly. Inoculation of chickens with the HPAIV A/H5N8 demonstrated neuroinvasiveness, multiorgan failure, and death of chickens on the 3rd day post inoculation. Virus replicated in all collected organ samples in high viral titers with the highest titer in the brain (6.75±0.1 log10 TCID50/ml). Effective virus replication was found in the following cells: neurons and glial cells of a brain; alveolar cells and macrophages of lungs; epithelial cells of a small intestine; hepatocytes and Kupffer cells of a liver; macrophages and endothelial cells of a spleen; and the tubular epithelial cells of kidneys. These findings advance our understanding of histopathological effect of A(H5N8) HPAIV infection.

Original languageEnglish
Article number4124865
Pages (from-to)4124865
JournalInterdisciplinary Perspectives on Infectious Diseases
Publication statusPublished - 1 Jan 2019


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