Novel cancer stem marker and its applicability for grading primary human gliomas

Evgeniya V. Dolgova, Sergey V. Mishinov, Anastasiya S. Proskurina, Ekaterina A. Potter, Yaroslav R. Efremov, Sergey I. Bayborodin, Tamara V. Tyrinova, Vyacheslav V. Stupak, Alexandr A. Ostatin, Elena R. Chernykh, Sergey S. Bogachev

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability to natively internalize fragmented double-stranded DNA. Using this feature as a marker, we show that 0.1% to 6% of human glioblastoma cells from the bioptates can effectively internalize a fluorescently labeled DNA probe. Of these, using samples from 3 patients, 66% to 100% cells are also positive for CD133, a well-established surface marker of tumor-initiating glioma stem cells. Using the samples from primary malignant brain lesions (33 patients), we demonstrate that tumor grading significantly correlates (R = .71) with the percentage of DNA-internalizing cells. No such correlation is observed for relapse samples (18 patients).

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalTechnology in Cancer Research and Treatment
Volume17
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • CD133
  • Glioblastoma
  • Neurosphere
  • TAMRA-DNA
  • Tumor-initiating stem cells
  • Humans
  • Neoplastic Stem Cells/metabolism
  • Brain Neoplasms/metabolism
  • AC133 Antigen
  • Biomarkers, Tumor
  • DNA, Neoplasm/genetics
  • Neoplasm Grading
  • Fluorescent Antibody Technique
  • Glioma/genetics
  • Tumor Cells, Cultured

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