New insights into phasmatodea chromosomes

Thomas Liehr, Olesya Buleu, Tatyana Karamysheva, Alexander Bugrov, Nikolai Rubtsov

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two Leptynia species. Interestingly, 10–25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (Medauroidea extradentata, Sungaya inexpectata, Sipyloidea sipylus, Phaenopharos khaoyaiensis, and Peruphasma schultei) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e., S. inexpectata, P. khaoyaiensis, and P. schultei. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea.

Original languageEnglish
Article number327
Number of pages15
JournalGenes
Volume8
Issue number11
DOIs
Publication statusPublished - 17 Nov 2017

Keywords

  • C-banding
  • Fluorescence in situ hybridization
  • Interstitial telomeric sequences
  • Phasmatodea
  • Ribosomal deoxyribonucleic acid
  • Stick insects
  • Telomeric repeats
  • B-CHROMOSOMES
  • ribosomal deoxyribonucleic acid
  • TELOMERIC SEQUENCES
  • CLONOPSIS-GALLICA
  • INSECTA PHASMATODEA
  • ACRIDIDAE
  • SOREX-GRANARIUS SORICIDAE
  • stick insects
  • ORTHOPTERA
  • fluorescence in situ hybridization
  • ORGANIZATION
  • EVOLUTION
  • telomeric repeats
  • DNA
  • interstitial telomeric sequences

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