Molecular determinants possibly involved in the adaptation of pandemic A(H1N1)09 influenza virus to a new host

Elena Prokopyeva, Alesia Romanovskaya, Kirill Sharshov, Ivan Sobolev, Alexander Alekseev, Alexander Durymanov, Alexander Shestopalov

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To determine the molecular basis of A(H1N1)pdm09 virus adaptation to a new host. Methods: The pandemic virus A/Russia/01/2009 was adapted to mice by serial lung-to-lung passages. The growth properties, pathogenicity and genome sequences of the mouse-adapted virus were compared with those of the parental strain. Results: The A(H1N1)pdm09 virus caused mouse death after only six lung-to-lung passages. Mutations in the influenza virus antigen were found in the brain, liver, kidney and in intestine by immunohistochemistry. Conclusion: Our findings suggest that multiple mutations in the viral genome promote rapid adaptation of the A(H1N1)pdm09 virus and cause generalized infection in mice.

Original languageEnglish
Pages (from-to)491-498
Number of pages8
JournalFuture Virology
Volume12
Issue number9
DOIs
Publication statusPublished - Sep 2017

Keywords

  • A(H1N1)pdm09 virus adaptation
  • HA
  • IHC mice model
  • NA
  • PA
  • PB1
  • PB2

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