Modular lentiviral vector system for chimeric antigen receptor design optimization

S. V. Kulemzin, N. A. Chikaev, O. Y. Volkova, V. V. Kuznetsova, A. V. Taranin, A. A. Gorchakov

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The article reports the development of a collection of lentiviral vector constructs enabling time-efficient production and testing of different variants of chimeric antigen receptors (CAR). These artificial surface proteins make it possible to redirect the activity of immune cytotoxic T-cells towards cancer cells. Chimeric antigen receptors usually encompass four functional modules, namely, antigen recognition, flexible linker, transmembrane, and signal modules. The use of modules with different properties allows modulating the affinity and specificity of CAR interaction with target antigens, as well as intensity and quality of activation signaling, which determines the cytotoxic properties of CAR T-cells, as well as their proliferation rate and time of persistence in the organism. The proposed vector system make it possible to easily test various combinations of CAR modules while its being open to distribution allows the direct comparison of the results obtained by different scientific groups.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalRussian Journal of Bioorganic Chemistry
Volume43
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • chimeric antigen receptors
  • lentiviral vector constructs

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