Mechanical properties and biological behavior of 3D matrices produced by electrospinning from protein-enriched polyurethane

Vera S. Chernonosova, Alexander A. Gostev, Yun Gao, Yuriy A. Chesalov, Alexey V. Shutov, Evgeniy A. Pokushalov, Andrey A. Karpenko, Pavel P. Laktionov

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Properties of matrices manufactured by electrospinning from solutions of polyurethane Tecoflex EG-80A with gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The concentration of gelatin added to the electrospinning solution was shown to influence the mechanical properties of matrices: the dependence of matrix tensile strength on protein concentration is described by a bell-shaped curve and an increase in gelatin concentration added to the elasticity of the samples. SEM, FTIR spectroscopy, and mechanical testing demonstrate that incubation of matrices in phosphate buffer changes the structure of the fibers and alters the polyurethane-gelatin interactions, increasing matrix durability. The ability of the matrices to maintain adhesion and proliferation of human endothelial cells was studied. The results suggest that matrices made of 3% polyurethane solution with 15% gelatin (wt/wt) and treated with glutaraldehyde are the optimal variant for cultivation of endothelial cells.

Original languageEnglish
Article number1380606
Pages (from-to)1380606
Number of pages10
JournalBioMed Research International
Volume2018
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Elasticity
  • Endothelial Cells
  • Gelatin
  • Humans
  • Polyurethanes/chemistry
  • Tensile Strength
  • Tissue Engineering
  • GELATIN
  • TECOPHILIC/GELATIN NANOFIBERS
  • VASCULAR GRAFT
  • RELEASE
  • SURFACE-ROUGHNESS
  • HUMAN ENDOTHELIAL-CELLS
  • IN-VITRO
  • TISSUE ENGINEERING APPLICATIONS
  • DEGRADATION
  • SCAFFOLDS

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