Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich, N. V. Yunusova, M. N. Stakheeva, A. K. Somov, A. E. Frolova, N. A. Kiryushina, S. G. Afanasyev, A. E. Grigor’eva, P. P. Laktionov, I. V. Kondakova

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalBiochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • breast cancer
  • colorectal cancer
  • exosomes
  • flow cytometry
  • transmission electron microscopy

Fingerprint Dive into the research topics of 'Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients'. Together they form a unique fingerprint.

Cite this