Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich; N. V. Yunusova; M. N. Stakheeva; A. K. Somov; A. E. Frolova; N. A. Kiryushina; S. G. Afanasyev; A. E. Grigor’eva; P. P. Laktionov; I. V. Kondakova

doi:10.1134/S1990750817030106

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich, N. V. Yunusova, M. N. Stakheeva, A. K. Somov, A. E. Frolova, N. A. Kiryushina, S. G. Afanasyev, A. E. Grigor’eva, P. P. Laktionov, I. V. Kondakova

Section of Molecular Biology and Biotechnology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.

Original language	English
Pages (from-to)	291-295
Number of pages	5
Journal	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Volume	11
Issue number	3
DOIs	https://doi.org/10.1134/S1990750817030106
Publication status	Published - 1 Jul 2017

Keywords

breast cancer
colorectal cancer
exosomes
flow cytometry
transmission electron microscopy

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10.1134/S1990750817030106

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Tamkovich, S. N., Yunusova, N. V., Stakheeva, M. N., Somov, A. K., Frolova, A. E., Kiryushina, N. A., Afanasyev, S. G., Grigor’eva, A. E., Laktionov, P. P., & Kondakova, I. V. (2017). Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 11(3), 291-295. https://doi.org/10.1134/S1990750817030106

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abstract = "A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.",

keywords = "breast cancer, colorectal cancer, exosomes, flow cytometry, transmission electron microscopy",

author = "Tamkovich, {S. N.} and Yunusova, {N. V.} and Stakheeva, {M. N.} and Somov, {A. K.} and Frolova, {A. E.} and Kiryushina, {N. A.} and Afanasyev, {S. G.} and Grigor{\textquoteright}eva, {A. E.} and Laktionov, {P. P.} and Kondakova, {I. V.}",

year = "2017",

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doi = "10.1134/S1990750817030106",

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Tamkovich, SN, Yunusova, NV, Stakheeva, MN, Somov, AK, Frolova, AE, Kiryushina, NA, Afanasyev, SG, Grigor’eva, AE, Laktionov, PP & Kondakova, IV 2017, 'Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients', Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, vol. 11, no. 3, pp. 291-295. https://doi.org/10.1134/S1990750817030106

TY - JOUR

T1 - Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

AU - Tamkovich, S. N.

AU - Yunusova, N. V.

AU - Stakheeva, M. N.

AU - Somov, A. K.

AU - Frolova, A. E.

AU - Kiryushina, N. A.

AU - Afanasyev, S. G.

AU - Grigor’eva, A. E.

AU - Laktionov, P. P.

AU - Kondakova, I. V.

PY - 2017/7/1

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N2 - A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.

AB - A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.

KW - breast cancer

KW - colorectal cancer

KW - exosomes

KW - flow cytometry

KW - transmission electron microscopy

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JF - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry

SN - 1990-7508

IS - 3

ER -

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

Abstract

Keywords

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