Intrachromosomal rearrangements in rodents from the perspective of comparative region-specific painting

Svetlana A. Romanenko, Natalya A. Serdyukova, Polina L. Perelman, Svetlana V. Pavlova, Nina S. Bulatova, Feodor N. Golenishchev, Roscoe Stanyon, Alexander S. Graphodatsky

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

It has long been hypothesized that chromosomal rearrangements play a central role in different evolutionary processes, particularly in speciation and adaptation. Interchromosomal rearrangements have been extensively mapped using chromosome painting. However, intrachromosomal rearrangements have only been described using molecular cytogenetics in a limited number of mammals, including a few rodent species. This situation is unfortunate because intrachromosomal rearrangements are more abundant than interchromosomal rearrangements and probably contain essential phylogenomic information. Significant progress in the detection of intrachromosomal rearrangement is now possible, due to recent advances in molecular biology and bioinformatics. We investigated the level of intrachromosomal rearrangement in the Arvicolinae subfamily, a species-rich taxon characterized by very high rate of karyotype evolution. We made a set of region specific probes by microdissection for a single syntenic region represented by the p-arm of chromosome 1 of Alexandromys oeconomus, and hybridized the probes onto the chromosomes of four arvicolines (Microtus agrestis, Microtus arvalis, Myodes rutilus, andDicrostonyx torquatus). These experiments allowed us to show the intrachromosomal rearrangements in the subfamily at a significantly higher level of resolution than previously described. We found a number of paracentric inversions in the karyotypes of M. agrestis and M. rutilus, as well as multiple inversions and a centromere shift in the karyotype of M. arvalis. We propose that during karyotype evolution, arvicolines underwent a significant number of complex intrachromosomal rearrangements that were not previously detected.

Original languageEnglish
Article number215
Number of pages10
JournalGenes
Volume8
Issue number9
DOIs
Publication statusPublished - 30 Aug 2017

Keywords

  • Centromere shift
  • Chromosome painting
  • Comparative cytogenetics
  • Fluorescence in situ hybridization
  • Inversion
  • Microdissection
  • Small mammals
  • Voles
  • MOLECULAR CYTOGENETIC ANALYSIS
  • X-CHROMOSOME
  • IN-SITU HYBRIDIZATION
  • comparative cytogenetics
  • inversion
  • SPECIATION
  • MAMMALIAN CHROMOSOME
  • KARYOTYPE EVOLUTION
  • FIELD VOLE
  • GENOME HOMOLOGY
  • fluorescence in situ hybridization
  • chromosome painting
  • voles
  • microdissection
  • ARVICOLINAE CRICETIDAE
  • centromere shift
  • small mammals
  • GENUS MICROTUS

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