Impact of PARP1, PARP2 & PARP3 on the Base Excision Repair of Nucleosomal DNA

M. M. Kutuzov, E. A. Belousova, E. S. Ilina, O. I. Lavrik

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

1 Citation (Scopus)

Abstract

DNA is constantly attacked by different damaging agents; therefore, it requires frequent repair. On the one hand, the base excision repair (BER) system is responsible for the repair of the most frequent DNA lesions. On the other hand, the formation of poly(ADP-ribose) is one of the main DNA damage response reactions that is catalysed by members of the PARP family. PARP1, which belongs to the PARP family and performs approximately 90% of PAR synthesis in cells, could be considered a main regulator of the BER process. Most of the experimental data concerning BER investigation have been obtained using naked DNA. However, in the context of the eukaryotic cell, DNA is compacted in the nucleus, and the lowest compaction level is represented by the nucleosome. Thus, the organization of DNA into the nucleosome impacts the DNA-protein interactions that are involved in BER processes. Poly(ADP-ribosyl)ation (PARylation) is thought to regulate the initiation of the BER process at the chromatin level. In this review, we focus on the mechanisms involved in BER in the nucleosomal context and the potential effect of PARylation, which is catalysed by DNA-dependent PARP1, PARP2 and PARP3 proteins, on this process.

Original languageEnglish
Title of host publicationMechanisms of Genome Protection and Repair
EditorsDmitry O. Zharkov
PublisherSpringer, Cham
Chapter4
Pages47-57
Number of pages11
Volume1241
ISBN (Electronic)978-3-030-41283-8
ISBN (Print)978-3-030-41282-1, 978-3-030-41285-2
DOIs
Publication statusPublished - 8 May 2020

Publication series

NameAdvances in experimental medicine and biology
ISSN (Print)0065-2598

Keywords

  • Base excision repair
  • DNA damge response
  • NCP
  • Nucleosome core particle
  • PARP1
  • PARP2
  • PARP3
  • Poly(ADP-ribosyl)ation
  • Poly(ADP-ribose) Polymerases/metabolism
  • Humans
  • DNA/genetics
  • Animals
  • DNA Repair
  • DNA Damage
  • Nucleosomes/genetics

OECD FOS+WOS

  • 1.06 BIOLOGICAL SCIENCES

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