High-frequency pulsed electron-electron double resonance spectroscopy on DNA duplexes using trityl tags and shaped microwave pulses

A. Kuzhelev, D. Akhmetzyanov, V. Denysenkov, G. Shevelev, O. Krumkacheva, E. Bagryanskaya, T. Prisner

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Accurate distances between two trityl paramagnetic tags site-specifically attached to DNA duplexes were measured by pulsed electron-electron double resonance spectroscopy at 180 and 260 GHz microwave frequencies. Up to a threefold increase in the sensitivity of 260 GHz PELDOR measurements was achieved by using shaped broad-band microwave pulses.

Original languageEnglish
Pages (from-to)26140-26144
Number of pages5
JournalPhysical Chemistry Chemical Physics
Volume20
Issue number41
DOIs
Publication statusPublished - 7 Nov 2018

Keywords

  • DNA/chemistry
  • Electron Spin Resonance Spectroscopy
  • Microwaves
  • Molecular Dynamics Simulation
  • Nucleic Acid Conformation
  • Temperature
  • Trityl Compounds/chemistry
  • ROOM-TEMPERATURE
  • NANOMETER-DISTANCE MEASUREMENTS
  • BAND INVERSION
  • NUCLEIC-ACIDS
  • PHYSIOLOGICAL TEMPERATURES
  • TRIARYLMETHYL RADICALS
  • DIPOLAR SPECTROSCOPY
  • STRUCTURAL EPR
  • SPIN LABELS
  • EPR SPECTROSCOPY

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