Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots

S. V. Gerasimova, E. K. Khlestkina, A. V. Kochetov, V. K. Shumny

Research output: Contribution to journalReview articlepeer-review

4 Citations (Scopus)

Abstract

Genome editing is a new methodology for DNA modification that has been developing in recent years. This review compares proposed methods of optimization and development of a modern genome editing system—CRISPR/Cas9—in monocots. Methodical approaches for in silico selecting target sites, designing an expression vector, transferring the vector expression cassette into plant cells, evaluating the results of the editing and nonspecific activity of the system, and obtaining modified plants free of foreign DNA are reviewed. The problem of legislative regulation and the prospects for using this method for commercial purposes are discussed.

Original languageEnglish
Pages (from-to)141-155
Number of pages15
JournalRussian Journal of Plant Physiology
Volume64
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • cereals
  • CRISPR/Cas9
  • crops
  • gene knockout
  • genome editing
  • genome engineering
  • GMOs
  • transgenic plants

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