Abstract
Genome editing is a new methodology for DNA modification that has been developing in recent years. This review compares proposed methods of optimization and development of a modern genome editing system—CRISPR/Cas9—in monocots. Methodical approaches for in silico selecting target sites, designing an expression vector, transferring the vector expression cassette into plant cells, evaluating the results of the editing and nonspecific activity of the system, and obtaining modified plants free of foreign DNA are reviewed. The problem of legislative regulation and the prospects for using this method for commercial purposes are discussed.
Original language | English |
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Pages (from-to) | 141-155 |
Number of pages | 15 |
Journal | Russian Journal of Plant Physiology |
Volume | 64 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Mar 2017 |
Keywords
- cereals
- CRISPR/Cas9
- crops
- gene knockout
- genome editing
- genome engineering
- GMOs
- transgenic plants