Genetic diversity of brucella melitensis in kazakhstan in relation to world-wide diversity

Elena Shevtsova, Gilles Vergnaud, Alexandr Shevtsov, Alexandr Shustov, Kalysh Berdimuratova, Kasim Mukanov, Marat Syzdykov, Andrey Kuznetsov, Larissa Lukhnova, Uinkul Izbanova, Maxim Filipenko, Yerlan Ramankulov

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We describe the genetic diversity of 1327 Brucella strains from human patients in Kazakhstan using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA). All strains were assigned to the Brucella melitensis East Mediterranean group and clustered into 16 MLVA11 genotypes, nine of which are reported for the first time. MLVA11 genotype 116 predominates (86.8%) and is present all over Kazakhstan indicating existence and temporary preservation of a “founder effect” among B. melitensis strains circulating in Central Eurasia. The diversity pattern observed in humans is highly similar to the pattern previously reported in animals. The diversity observed by MLVA suggested that the epidemiological status of brucellosis in Kazakhstan is the result of the introduction of a few lineages, which have subsequently diversified at the most unstable tandem repeat loci. This investigation will allow to select the most relevant strains for testing these hypotheses via whole genome sequencing and to subsequently adjust the genotyping scheme to the Kazakhstan epidemiological situation.

Original languageEnglish
Article number1897
Pages (from-to)1897
Number of pages11
JournalFrontiers in Microbiology
Volume10
Issue numberAUG
DOIs
Publication statusPublished - 13 Aug 2019

Keywords

  • Brucella melitensis
  • Genetic diversity
  • Genotyping
  • Kazakhstan
  • Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA)
  • genetic diversity
  • MULTIPLEX PCR
  • ASSAY
  • ASIA
  • genotyping
  • multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA)

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