Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)

E. V. Grigor'eva; T. B. Malankhanova; E. I. Ustyantseva; J. M. Minina; O. E. Redina; V. V. Morozov; A. I. Shevela; S. M. Zakian; S. P. Medvedev

doi:10.1016/j.scr.2019.101415

Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)

E. V. Grigor'eva, T. B. Malankhanova, E. I. Ustyantseva, J. M. Minina, O. E. Redina, V. V. Morozov, A. I. Shevela, S. M. Zakian, S. P. Medvedev

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Abstract

The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening.

Original language	English
Article number	101415
Number of pages	5
Journal	Stem Cell Research
Volume	36
DOIs	https://doi.org/10.1016/j.scr.2019.101415
Publication status	Published - 1 Apr 2019

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Grigor'eva, E. V., Malankhanova, T. B., Ustyantseva, E. I., Minina, J. M., Redina, O. E., Morozov, V. V., Shevela, A. I., Zakian, S. M., & Medvedev, S. P. (2019). Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I). Stem Cell Research, 36, [101415]. https://doi.org/10.1016/j.scr.2019.101415

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title = "Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)",

abstract = "The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening.",

author = "Grigor'eva, {E. V.} and Malankhanova, {T. B.} and Ustyantseva, {E. I.} and Minina, {J. M.} and Redina, {O. E.} and Morozov, {V. V.} and Shevela, {A. I.} and Zakian, {S. M.} and Medvedev, {S. P.}",

year = "2019",

month = apr,

day = "1",

doi = "10.1016/j.scr.2019.101415",

language = "English",

volume = "36",

journal = "Stem Cell Research",

issn = "1873-5061",

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}

Grigor'eva, EV, Malankhanova, TB, Ustyantseva, EI, Minina, JM, Redina, OE, Morozov, VV, Shevela, AI, Zakian, SM & Medvedev, SP 2019, 'Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)', Stem Cell Research, vol. 36, 101415. https://doi.org/10.1016/j.scr.2019.101415

TY - JOUR

T1 - Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)

AU - Grigor'eva, E. V.

AU - Malankhanova, T. B.

AU - Ustyantseva, E. I.

AU - Minina, J. M.

AU - Redina, O. E.

AU - Morozov, V. V.

AU - Shevela, A. I.

AU - Zakian, S. M.

AU - Medvedev, S. P.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening.

AB - The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening.

UR - http://www.scopus.com/inward/record.url?scp=85062390628&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2019.101415

DO - 10.1016/j.scr.2019.101415

M3 - Article

C2 - 30851551

AN - SCOPUS:85062390628

VL - 36

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 101415

ER -

Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I)

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