Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

E. V. Grigor'eva; T. B. Malankhanova; A. Surumbayeva; J. M. Minina; V. V. Morozov; N. Yu Abramycheva; S. N. Illarioshkin; A. A. Malakhova; S. M. Zakian

doi:10.1016/j.scr.2018.101382

Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

E. V. Grigor'eva, T. B. Malankhanova, A. Surumbayeva, J. M. Minina, V. V. Morozov, N. Yu Abramycheva, S. N. Illarioshkin, A. A. Malakhova, S. M. Zakian

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by mutation in the HTT gene encoding HTT protein. The mutant protein leads to the neuronal death through dysregulation of multiple cellular processes. HD human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study. iPSC line from HD patient with 47 CAG repeats in HTT was generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC line retained the mutation, expressed pluripotency markers, had a normal karyotype and displayed in vitro differentiation to the three germ layers.

Original language	English
Article number	101382
Number of pages	5
Journal	Stem Cell Research
Volume	34
DOIs	https://doi.org/10.1016/j.scr.2018.101382
Publication status	Published - 1 Jan 2019

Keywords

Adult
Cell Culture Techniques/methods
Cell Line
Cellular Reprogramming
Female
Humans
Huntington Disease/blood
Induced Pluripotent Stem Cells/pathology
Leukocytes, Mononuclear/pathology

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Grigor'eva, E. V., Malankhanova, T. B., Surumbayeva, A., Minina, J. M., Morozov, V. V., Abramycheva, N. Y., Illarioshkin, S. N., Malakhova, A. A., & Zakian, S. M. (2019). Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease. Stem Cell Research, 34, [101382]. https://doi.org/10.1016/j.scr.2018.101382

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title = "Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease",

abstract = "Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by mutation in the HTT gene encoding HTT protein. The mutant protein leads to the neuronal death through dysregulation of multiple cellular processes. HD human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study. iPSC line from HD patient with 47 CAG repeats in HTT was generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC line retained the mutation, expressed pluripotency markers, had a normal karyotype and displayed in vitro differentiation to the three germ layers.",

keywords = "Adult, Cell Culture Techniques/methods, Cell Line, Cellular Reprogramming, Female, Humans, Huntington Disease/blood, Induced Pluripotent Stem Cells/pathology, Leukocytes, Mononuclear/pathology",

author = "Grigor'eva, {E. V.} and Malankhanova, {T. B.} and A. Surumbayeva and Minina, {J. M.} and Morozov, {V. V.} and Abramycheva, {N. Yu} and Illarioshkin, {S. N.} and Malakhova, {A. A.} and Zakian, {S. M.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = jan,

day = "1",

doi = "10.1016/j.scr.2018.101382",

language = "English",

volume = "34",

journal = "Stem Cell Research",

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Grigor'eva, EV, Malankhanova, TB, Surumbayeva, A, Minina, JM, Morozov, VV, Abramycheva, NY, Illarioshkin, SN, Malakhova, AA & Zakian, SM 2019, 'Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease', Stem Cell Research, vol. 34, 101382. https://doi.org/10.1016/j.scr.2018.101382

TY - JOUR

T1 - Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

AU - Grigor'eva, E. V.

AU - Malankhanova, T. B.

AU - Surumbayeva, A.

AU - Minina, J. M.

AU - Morozov, V. V.

AU - Abramycheva, N. Yu

AU - Illarioshkin, S. N.

AU - Malakhova, A. A.

AU - Zakian, S. M.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by mutation in the HTT gene encoding HTT protein. The mutant protein leads to the neuronal death through dysregulation of multiple cellular processes. HD human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study. iPSC line from HD patient with 47 CAG repeats in HTT was generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC line retained the mutation, expressed pluripotency markers, had a normal karyotype and displayed in vitro differentiation to the three germ layers.

AB - Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by mutation in the HTT gene encoding HTT protein. The mutant protein leads to the neuronal death through dysregulation of multiple cellular processes. HD human induced pluripotent stem cells (iPSCs) represent a useful and valid model for the disease study. iPSC line from HD patient with 47 CAG repeats in HTT was generated from blood mononuclear cells by non-integrating episomal vectors. The iPSC line retained the mutation, expressed pluripotency markers, had a normal karyotype and displayed in vitro differentiation to the three germ layers.

KW - Adult

KW - Cell Culture Techniques/methods

KW - Cell Line

KW - Cellular Reprogramming

KW - Female

KW - Humans

KW - Huntington Disease/blood

KW - Induced Pluripotent Stem Cells/pathology

KW - Leukocytes, Mononuclear/pathology

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DO - 10.1016/j.scr.2018.101382

M3 - Article

C2 - 30658253

AN - SCOPUS:85059966398

VL - 34

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 101382

ER -

Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease

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