Functional study of potential regulatory SNPs (rs590352, rs11542583, rs3829202, rs78317230, rs2072580, rs4796672)

Arina Degtyareva, Elena Leberfarb, Ilya Brusentsov, Tatiana Kuzina, Tatiana Merkulova

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

In a previous study using a new bioinformatic approach based on Chip-Seq data with antibodies against various transcription factors and histone modifications, ChiA-PET, RNA-seq and ICGC data, 32 potentially regulatory single - nucleotide polymorphisms (rSNPs) were associated with colorectal cancer. In this study, a functional analysis of 6 of these polymorphisms was performed using EMSA and the luciferase reporter system. These nucleotide substitutions have been shown to alter the binding of nuclear extract proteins and influence the expression of the reporter gene.

Original languageEnglish
Title of host publicationProceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages122-125
Number of pages4
ISBN (Electronic)9781728195971
DOIs
Publication statusPublished - Jul 2020
Event2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020 - Novosibirsk, Russian Federation
Duration: 6 Jul 202010 Jul 2020

Publication series

NameProceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

Conference

Conference2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
CountryRussian Federation
CityNovosibirsk
Period06.07.202010.07.2020

Keywords

  • Electrophoretic Mobility Shift Assay (EMSA)
  • gene expression
  • luciferase assay
  • regulatory SNP (rSNP)

OECD FOS+WOS

  • 1.02 COMPUTER AND INFORMATION SCIENCES
  • 1.06 BIOLOGICAL SCIENCES
  • 3.01 BASIC MEDICAL RESEARCH
  • 5.09 OTHER SOCIAL SCIENCES

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