Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev; Ekaterina V. Bondina; Sergey A. Dolgushin; Victoria S. Semeykina; Natalya N. Sankova; Ekaterina V. Parkhomchuk

doi:10.1117/12.2526387

Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, Ekaterina V. Parkhomchuk

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review

Abstract

Samples of liquid dispersions of 4 µm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

Original language	English
Title of host publication	European Conference on Biomedical Optics, ECBO_2019
Editors	JC Brown, TG VanLeeuwen
Publisher	OSA - The Optical Society
Number of pages	3
ISBN (Electronic)	9781557528209
DOIs	https://doi.org/10.1117/12.2526387
Publication status	Published - 19 Jul 2019
Event	European Conference on Biomedical Optics, ECBO_2019 - Munich, Netherlands Duration: 23 Jun 2019 → 25 Jun 2019

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F142-ECBO 2019

Conference

Conference	European Conference on Biomedical Optics, ECBO_2019
Country	Netherlands
City	Munich
Period	23.06.2019 → 25.06.2019

Keywords

Cyanine dyes
Immunofluorescence analysis
Polystyrene microspheres
polystyrene microspheres
immunofluorescence analysis
cyanine dyes

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10.1117/12.2526387

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Shalaev, P. V., Bondina, E. V., Dolgushin, S. A., Semeykina, V. S., Sankova, N. N., & Parkhomchuk, E. V. (2019). Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. In JC. Brown, & TG. VanLeeuwen (Eds.), European Conference on Biomedical Optics, ECBO_2019 (Optics InfoBase Conference Papers; Vol. Part F142-ECBO 2019). OSA - The Optical Society. https://doi.org/10.1117/12.2526387

Shalaev, Pavel V. ; Bondina, Ekaterina V. ; Dolgushin, Sergey A. ; Semeykina, Victoria S. ; Sankova, Natalya N. ; Parkhomchuk, Ekaterina V. / Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. European Conference on Biomedical Optics, ECBO_2019. editor / JC Brown ; TG VanLeeuwen. OSA - The Optical Society, 2019. (Optics InfoBase Conference Papers).

@inproceedings{2e2cc09bd57d46ce9cb90382f8b9af85,

title = "Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis",

abstract = "Samples of liquid dispersions of 4 µm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.",

keywords = "Cyanine dyes, Immunofluorescence analysis, Polystyrene microspheres, polystyrene microspheres, immunofluorescence analysis, cyanine dyes",

author = "Shalaev, {Pavel V.} and Bondina, {Ekaterina V.} and Dolgushin, {Sergey A.} and Semeykina, {Victoria S.} and Sankova, {Natalya N.} and Parkhomchuk, {Ekaterina V.}",

year = "2019",

month = jul,

day = "19",

doi = "10.1117/12.2526387",

language = "English",

series = "Optics InfoBase Conference Papers",

publisher = "OSA - The Optical Society",

editor = "JC Brown and TG VanLeeuwen",

booktitle = "European Conference on Biomedical Optics, ECBO_2019",

note = "European Conference on Biomedical Optics, ECBO_2019 ; Conference date: 23-06-2019 Through 25-06-2019",

}

Shalaev, PV, Bondina, EV, Dolgushin, SA, Semeykina, VS, Sankova, NN & Parkhomchuk, EV 2019, Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. in JC Brown & TG VanLeeuwen (eds), European Conference on Biomedical Optics, ECBO_2019. Optics InfoBase Conference Papers, vol. Part F142-ECBO 2019, OSA - The Optical Society, European Conference on Biomedical Optics, ECBO_2019, Munich, Netherlands, 23.06.2019. https://doi.org/10.1117/12.2526387

Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. / Shalaev, Pavel V.; Bondina, Ekaterina V.; Dolgushin, Sergey A.; Semeykina, Victoria S.; Sankova, Natalya N.; Parkhomchuk, Ekaterina V.

European Conference on Biomedical Optics, ECBO_2019. ed. / JC Brown; TG VanLeeuwen. OSA - The Optical Society, 2019. (Optics InfoBase Conference Papers; Vol. Part F142-ECBO 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review

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T1 - Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

AU - Shalaev, Pavel V.

AU - Bondina, Ekaterina V.

AU - Dolgushin, Sergey A.

AU - Semeykina, Victoria S.

AU - Sankova, Natalya N.

AU - Parkhomchuk, Ekaterina V.

PY - 2019/7/19

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AB - Samples of liquid dispersions of 4 µm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

KW - Cyanine dyes

KW - Immunofluorescence analysis

KW - Polystyrene microspheres

KW - polystyrene microspheres

KW - immunofluorescence analysis

KW - cyanine dyes

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Shalaev PV, Bondina EV, Dolgushin SA, Semeykina VS, Sankova NN , Parkhomchuk EV. Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. In Brown JC, VanLeeuwen TG, editors, European Conference on Biomedical Optics, ECBO_2019. OSA - The Optical Society. 2019. (Optics InfoBase Conference Papers). https://doi.org/10.1117/12.2526387