Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, Ekaterina V. Parkhomchuk

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

Samples of liquid dispersions of 4 µm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO_2019
EditorsJC Brown, TG VanLeeuwen
PublisherOSA - The Optical Society
Number of pages3
ISBN (Electronic)9781557528209
DOIs
Publication statusPublished - 19 Jul 2019
EventEuropean Conference on Biomedical Optics, ECBO_2019 - Munich, Netherlands
Duration: 23 Jun 201925 Jun 2019

Publication series

NameOptics InfoBase Conference Papers
VolumePart F142-ECBO 2019

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO_2019
CountryNetherlands
CityMunich
Period23.06.201925.06.2019

Keywords

  • Cyanine dyes
  • Immunofluorescence analysis
  • Polystyrene microspheres
  • polystyrene microspheres
  • immunofluorescence analysis
  • cyanine dyes

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    Shalaev, P. V., Bondina, E. V., Dolgushin, S. A., Semeykina, V. S., Sankova, N. N., & Parkhomchuk, E. V. (2019). Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. In JC. Brown, & TG. VanLeeuwen (Eds.), European Conference on Biomedical Optics, ECBO_2019 (Optics InfoBase Conference Papers; Vol. Part F142-ECBO 2019). OSA - The Optical Society. https://doi.org/10.1117/12.2526387