Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, Ekaterina V. Parkhomchuk

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

Original languageEnglish
Title of host publicationClinical and Preclinical Optical Diagnostics II
EditorsJ. Quincy Brown, Ton G. van Leeuwen
PublisherSPIE
Number of pages3
ISBN (Electronic)9781510628397
DOIs
Publication statusPublished - 1 Jan 2019
EventClinical and Preclinical Optical Diagnostics II 2019 - Munich, Germany
Duration: 23 Jun 201925 Jun 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11073
ISSN (Print)1605-7422

Conference

ConferenceClinical and Preclinical Optical Diagnostics II 2019
CountryGermany
CityMunich
Period23.06.201925.06.2019

Keywords

  • Cyanine dyes
  • Immunofluorescence analysis
  • Polystyrene microspheres
  • polystyrene microspheres
  • immunofluorescence analysis
  • cyanine dyes

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    Shalaev, P. V., Bondina, E. V., Dolgushin, S. A., Semeykina, V. S., Sankova, N. N., & Parkhomchuk, E. V. (2019). Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. In J. Q. Brown, & T. G. van Leeuwen (Eds.), Clinical and Preclinical Optical Diagnostics II [110731W] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11073). SPIE. https://doi.org/10.1117/12.2526387