Fluorescent bacterial biosensor E. Coli/pTdcR-TurboYFP sensitive to terahertz radiation

Danil S. Serdyukov, Tatiana N. Goryachkovskaya, Irina A. Mescheryakova, Sergei A. Kuznetsov, Vasiliy M. Popik, Sergey E. Peltek

Research output: Contribution to journalArticlepeer-review

Abstract

A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli (E. coli) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli.

Original languageEnglish
Pages (from-to)705-721
Number of pages17
JournalBiomedical Optics Express
Volume12
Issue number2
DOIs
Publication statusPublished - 2021

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