Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro

Tatiana S. Kalinina, Vladislav V. Kononchuk, Vladimir Y. Ovchinnikov, Mikhail D. Chanyshev, Lyudmila F. Gulyaeva

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.

Original languageEnglish
Pages (from-to)1937-1945
Number of pages9
JournalMolecular Biology Reports
Volume45
Issue number6
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Constitutive androstane receptor
  • Dichlorodiphenyltrichloroethane
  • Estrogen receptor
  • MicroRNA
  • Xenobiotics
  • PROFILES
  • SMALL RNAS
  • TUMORS
  • PESTICIDES
  • BREAST-CANCER
  • ROLES
  • SAMPLES
  • RECEPTORS
  • POLYCHLORINATED-BIPHENYLS
  • MICRORNA

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