Ellipsometry, Raman spectroscopy and SOI- nanowire biosensor in diagnosis of colorectalcancer

M. V. Kruchinina, Ya I. Prudnikova, S. A. Kurilovich, A. A. Gromov, V. N. Kruchinin, V. V. Atuchin, O. V. Naumova, E. V. Spesivtsev, V. A. Volodin, S. E. Peltek, G. V. Shuvalov, V. M. Generalov

Research output: Contribution to journalArticlepeer-review

Abstract

Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001-0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13-10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.

Original languageEnglish
Pages (from-to)32-41
Number of pages10
JournalSiberian Journal of Oncology
Volume16
Issue number4
DOIs
Publication statusPublished - 2017

Keywords

  • Colorectal cancer
  • Diagnosis
  • Ellipsometry
  • Fluorescence optical spectrometry
  • Raman spectroscopy
  • SOI nanowire biosensor
  • Surface Plasmon resonance
  • Tumor M2 pyruvate kinase

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