Effects of Three-time Administration of a Supramolecular Complex of Praziquantel with Disodium Glycyrrhizinate on Trematode Opisthorchis felineus in Hamsters

Damira Avgustinovich, Maria Lvova, Galina Vishnivetskaya, Mikhail Tsyganov, Irina Orlovskaya, Lyudmila Toporkova, Elena Goiman, Aleksander Dushkin, Nikolay Lyakhov, Viatcheslav Mordvinov

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Praziquantel (PZQ) is the most commonly used anthelmintic drug for treating trematodiases. It was shown here that PZQ in complex with disodium glycyrrhizinate (PZQ-Na2GA, in the 1:10 ratio) has higher bioavailability than PZQ alone. Our aim was to determine the effects of three-time administration of PZQ-Na2GA in an experimental opisthorchiasis felinea model. Methods: The PZQ-Na2GA complex (1:10) at a 400 mg/kg dose (meaning 36.4 mg/kg PZQ) was administered to Opisthorchis felineus-infected hamsters three times under a “9:00 am–6:00 pm–9:00 am” regimen (PZQ-Na2GA × 3). Effects of treatment were assessed as a reduction of helminth load in the hamsters and as changes in physiological, hematological, and blood biochemical parameters. The helminths extracted from the liver of the hamsters that received PZQ-Na2GA thrice were assayed for sensitivity to PZQ in vitro. Results: PZQ-Na2GA × 3 reduced the number of O. felineus helminths in the liver by 87%, which is 30% better than a previously reported effect of one-time administration of the complex. Meanwhile, relative weights of the liver and thymus diminished, and some hematological parameters improved. The helminths extracted from the hamsters 1 month after the PZQ-Na2GA × 3 treatment showed elevated sensitivity to PZQ, as determined in vitro. Conclusion: Compared with previously published data on the effectiveness of various drugs in experimental opisthorchiasis felinea, PZQ-Na2GA × 3 exerts the most potent anthelmintic effect. In addition, PZQ-Na2GA × 3 improves physiological status of O. felineus-infected hamsters and sensitizes the surviving parasites to subsequent PZQ treatment.

Original languageEnglish
Number of pages8
JournalActa Parasitologica
Early online date8 Jan 2021
DOIs
Publication statusE-pub ahead of print - 8 Jan 2021

Keywords

  • Disodium glycyrrhizinate
  • Hamsters
  • In vitro
  • Opisthorchis felineus
  • Praziquantel
  • FIBROSIS
  • VIVERRINI
  • ACID
  • INFECTION
  • EXPRESSION
  • CLONORCHIASIS

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