Effects of slow freezing and vitrification on embryo development in domestic cat

Valentina I. Mokrousova, Konstantin A. Okotrub, Eugeny Y. Brusentsev, Elena A. Kizilova, Nikolai V. Surovtsev, Sergei Y. Amstislavsky

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Cryopreservation of gametes and embryos is used to maintain genetic diversity of domestic and wild felids. However, felid oocytes and preimplantation embryos contain large amount of intracellular lipids, which affect their cryosensitivity. The objective was to compare the effects of slow freezing and vitrification and to study lipid phase transition (LPT) during cooling in cat embryos. In vitro-derived embryos were cultured 48 hr up to 4–8 cell stage, thereafter were either slow frozen or vitrified. Propylene glycol (PG) alone was used as a cryoprotective agent (CPA) for slow freezing, and a mixture of PG and dimethyl sulfoxide (DMSO) were used as CPAs for vitrification. After thawing/warming, embryos were in vitro cultured additionally for 72 hr. The total time of in vitro culture was 120 hr for all the groups including non-frozen controls. Effects of both cryopreservation procedures on the subsequent embryo development and nuclear fragmentation rate in embryonic cells were compared. There was no significant differences among the percentages of embryos achieved morula and early blastocyst stage in frozen-thawed group (36.4% and 20.0%), in vitrified-warmed group (34.3% and 28.6%) and in controls (55.6% and 25.9%). Cell numbers as well as nuclear fragmentation rate did not differ in these three groups. Average lipid phase transition (LPT) temperature (T*) was found to be relatively low (–2.2 ± 1.3°C) for the domestic cat embryos. It is supposed that the low LPT of LDs may provide a good background for successful application of slow freezing to domestic cat embryos. Generally, our study indicates that slow freezing and vitrification are both applicable for domestic cat embryo cryopreservation.

Original languageEnglish
Pages (from-to)1328-1336
Number of pages9
JournalReproduction in Domestic Animals
Volume55
Issue number10
Early online date20 Jul 2020
DOIs
Publication statusPublished - 1 Oct 2020

Keywords

  • domestic cat
  • lipid phase transition
  • preimplantation embryos
  • Raman spectroscopy
  • slow freezing
  • vitrification
  • SURVIVAL
  • VIVO
  • CRYOPRESERVATION
  • IN-VITRO CULTURE
  • TECHNOLOGIES
  • INTEGRITY
  • PHASE-TRANSITION
  • PREIMPLANTATION EMBRYOS
  • OOCYTES
  • LIPIDS

OECD FOS+WOS

  • 4.01 AGRICULTURE, FORESTRY, FISHERIES
  • 4.03 VETERINARY SCIENCE
  • 1.06.WF REPRODUCTIVE BIOLOGY

Fingerprint

Dive into the research topics of 'Effects of slow freezing and vitrification on embryo development in domestic cat'. Together they form a unique fingerprint.

Cite this