Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

A. Ya Bolsunovsky, D. V. Dementyev, T. S. Frolova, E. A. Trofimova, E. M. Iniatkina, S. A. Vasilyev, O. I. Sinitsyna

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

Original languageEnglish
Pages (from-to)362-366
Number of pages5
JournalDoklady. Biochemistry and biophysics
Volume489
Issue number1
DOIs
Publication statusPublished - 1 Nov 2019

Keywords

  • DNA Damage
  • Dose-Response Relationship, Radiation
  • Gamma Rays/adverse effects
  • Onions/genetics
  • Seedlings/genetics
  • CELLS
  • REPAIR
  • COMET ASSAY

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