Abstract
Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.
Original language | English |
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Pages (from-to) | 362-366 |
Number of pages | 5 |
Journal | Doklady. Biochemistry and biophysics |
Volume | 489 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Nov 2019 |
Keywords
- DNA Damage
- Dose-Response Relationship, Radiation
- Gamma Rays/adverse effects
- Onions/genetics
- Seedlings/genetics
- CELLS
- REPAIR
- COMET ASSAY