Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

A. Ya Bolsunovsky; D. V. Dementyev; T. S. Frolova; E. A. Trofimova; E. M. Iniatkina; S. A. Vasilyev; O. I. Sinitsyna

doi:10.1134/S1607672919060024

Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

A. Ya Bolsunovsky, D. V. Dementyev, T. S. Frolova, E. A. Trofimova, E. M. Iniatkina, S. A. Vasilyev, O. I. Sinitsyna

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

Original language	English
Pages (from-to)	362-366
Number of pages	5
Journal	Doklady. Biochemistry and biophysics
Volume	489
Issue number	1
DOIs	https://doi.org/10.1134/S1607672919060024
Publication status	Published - 1 Nov 2019

Keywords

DNA Damage
Dose-Response Relationship, Radiation
Gamma Rays/adverse effects
Onions/genetics
Seedlings/genetics
CELLS
REPAIR
COMET ASSAY

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10.1134/S1607672919060024

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title = "Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings",

abstract = "Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.",

keywords = "DNA Damage, Dose-Response Relationship, Radiation, Gamma Rays/adverse effects, Onions/genetics, Seedlings/genetics, CELLS, REPAIR, COMET ASSAY",

author = "Bolsunovsky, {A. Ya} and Dementyev, {D. V.} and Frolova, {T. S.} and Trofimova, {E. A.} and Iniatkina, {E. M.} and Vasilyev, {S. A.} and Sinitsyna, {O. I.}",

year = "2019",

month = nov,

day = "1",

doi = "10.1134/S1607672919060024",

language = "English",

volume = "489",

pages = "362--366",

journal = "Doklady Biochemistry and Biophysics",

issn = "1607-6729",

publisher = "Maik Nauka-Interperiodica Publishing",

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TY - JOUR

T1 - Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

AU - Bolsunovsky, A. Ya

AU - Dementyev, D. V.

AU - Frolova, T. S.

AU - Trofimova, E. A.

AU - Iniatkina, E. M.

AU - Vasilyev, S. A.

AU - Sinitsyna, O. I.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

AB - Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

KW - DNA Damage

KW - Dose-Response Relationship, Radiation

KW - Gamma Rays/adverse effects

KW - Onions/genetics

KW - Seedlings/genetics

KW - CELLS

KW - REPAIR

KW - COMET ASSAY

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DO - 10.1134/S1607672919060024

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VL - 489

SP - 362

EP - 366

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - 1

ER -

Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

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Keywords

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